两株土壤分离菌的解磷能力及对玉米的促生作用  

Two phosphate-solubilizing bacteria from calcareous soil: Solubilizing-phosphate capacity and promoting effect on corn seedling growth

作　　者：段秀梅[1] 高晓蓉[1] 吕军[1] 安利佳[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116023

出　　处：《中国土壤与肥料》2010年第2期79-85,共7页Soil and Fertilizer Sciences in China

基　　金：辽宁省大连市科学技术基金项目计划优秀青年科技人才基金(2005J22JH044)资助

摘　　要：从植物根际的土壤样品中分离筛选出两株高效解磷细菌P9和P28,分别被鉴定为粘质沙雷氏菌和产气肠杆菌。研究了不同环境条件对解磷能力的影响,结果表明,P28耐3%NaCl（w/v,下同）、28℃是两菌的最适温度、在初始pH 5.5~10.0条件下培养时能够高效溶解Ca3（PO4）2。盆栽试验结果表明,单接种、双接种解磷菌处理的玉米株高、植株干重和磷含量显著高于不接菌对照;单接种P9处理的玉米明显强于单接种P28处理;将有机肥（M）和磷矿石（RP）作为载体和解磷菌一同混合施入土壤的处理,玉米苗干重,磷含量较单施解磷菌显著增加。Two efficient phosphate-solubilizing bacteria（PSBs）were isolated from plant rhizosphere soil samples,and were identified as Serratia marcescens P9and Enterobacter aerogenes P28.We measured quantitatively phosphate solubilization activities under various environmental conditions,28℃ was their optimum temperature,P28 was able to withstand high concentration of NaCl upto 3%（w/v,The same below）,and a wide range of initial pH from 5.5 to 10.0 while hydrolyzing phosphate compounds actively.Pot experiment showed that plant height,dry mass and phosphorus content was significantly higher with single-inoculation,dual inoculation PSBs treatments than CK;these indicators of corn only applying P9 agent were better than applying P28 agent only;Applying PSBs agent with manure and rock phosphate as a carrier could significantly increase the seedling＇dry weight,phosphorus accumulation,in comparing with applying PSBs agent alone.

关 键 词：解磷 鉴定 生物肥料 盆栽试验 

分 类 号：S513[农业科学—作物学] S154.3