对市售桑叶药材中芦丁的含量调查  

Investigation of the content of rutin in commercial mulberry leaves

作　　者：曾森[1] 刘虹[1] 张德芹[1] 刘韦鋆[1] 朱晓月[1] 郝彧[1] 

机构地区：[1]天津中医药大学中医药研究院教育部方剂学重点实验室,天津300193

出　　处：《中医药导报》2010年第4期87-88,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重大科技专项--"重大新药创制"(2009ZX09102-128)

摘　　要：目的:考察并分析桑叶药材的质量现状。方法:采用HPLC法分别对10份市售药材样品的芦丁含量进行测定。采用高效液相色谱法,Phenomsil C18(250mm×4.6mm)为色谱柱,以甲醇-0.5%磷酸溶液为流动相进行梯度洗脱,检测波长为358nm,柱温为24℃。结果:10份样品的芦丁含量均未能达到《中国药典》(2005年版)的含量标准。结论:桑叶中芦丁含量普遍较低。Objective： To determine the content of rutin in mulberry leaves from different areas and analyze their market situation. Methods： HPLC separation was achieved on Phenomsil Cls column （250 mm×4.6 mm） using gradient elution with mo- bile phase consisting of methanol and water containing 0.5% phosphoric acid. The elution was monitored at the wavelength of 358 nm, and the column was kept at 24 ℃. Results： All of 10 commercial samples did not reach standard of Chinese Phar- macopoeia. Conclusion： The content of rutin in mulberry leaves is generally low .

关 键 词：桑叶 芦丁 含量测定 

分 类 号：R927.2[医药卫生—药学]