野木瓜片中绿原酸的含量测定  

Determination of chlorogenic acid in Caulis stauntoniae cbinensis tablet

作　　者：李国强[1,2] 周本宏[1] 吴国秋[2] 张志坚[2] 

机构地区：[1]武汉大学,湖北武汉430072 [2]广东省广州市花城制药厂,广东广州510555

出　　处：《中国当代医药》2010年第10期114-115,共2页China Modern Medicine

摘　　要：目的:建立野木瓜片质量标准中的含量测定方法。方法:用HPLC法测定野木瓜片中绿原酸的含量,以十八烷基键合硅胶为固定相,乙腈:0.1%磷酸(体积比11:89)为流动相,流速为1ml/min,测定波长为327nm。结果:绿原酸在10.21～102.1μg/ml浓度范围内有良好的线性关系(r=1.0000),回收率为98.90%(RSD=0.8%)。结论:本法测定野木瓜片中的绿原酸含量,结果稳定、准确可靠,可作为该品种的质量控制方法。Objective：HPLC was used to determine the content of chlorogenic acid in Caulis stauntoniae cbinensis tablet.Methods：The study indicated that the effects of separation and determination were optimum when deter-mination parameters were as follows：analytic column：Shim-pack VP-ODS column,the mobile phase was acetonitrile-0.1% Phosphate （11：89）,The flow rate was 1 ml/min;UV detectionwavelength was 327 nm.Results：The repressive equation had a good linear relationship （r=1.000 0）.The relationship of injection amounts and peak was linear in the range of 10.21-102.1 μg/ml and recovery rate was 98.9%（RSD=0.8%）.The method was simple and available,accuracy and good reproducibility.Conclusion：This Caulis stauntoniae cbinensis tablet determination of chlorogenic acid content,the results are stable,accurate and reliable,can be used as the variety of quality control methods.

关 键 词：野木瓜片 绿原酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]