βCaMKII蛋白修饰转基因小鼠工作记忆的研究  

Study of βCaMKII on working memory in protein modified transgenic mice

作　　者：殷亮[1] 曾庆文[1] 杜丹[1] 曹晓华[1] 

机构地区：[1]华东师范大学上海市脑功能基因组学教育部重点实验室,上海200062

出　　处：《华东师范大学学报（自然科学版）》2010年第2期119-126,共8页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(30670682)

摘　　要：利用前额叶脑区过量表达β钙离子/钙调素依赖的蛋白激酶II(βCaMKII)的蛋白修饰转基因小鼠,研究βCaMKII对小鼠工作记忆的影响.Western Blot结果显示,转基因小鼠前额叶脑区的βCaMKII过量表达.在修饰的水迷宫实验中,与对照组相比,转基因小鼠的逃避潜伏期没有发生明显变化.此外,在T迷宫实验中,转基因小鼠的正确率与对照组的正确率也基本相同.由此推测,βCaMKII的过量表达对于前额叶皮层依赖的工作记忆没有明显的影响.This experiment was designed to investigate the effect of the β Ca2＋/calmodulin-dependent protein kinases II（βCaMKII） on the working memory in mice.The result of Western Blot showed that βCaMKII was overexpressed in the prefrontal cortex in transgenic mice.There was no significance in escape latency between transgenic mice and controls in Morris water maze.In addition,the correct of transgenic mice in T-maze was also similar to that of controls.These results suppose that overexpression of the βCaMKII has no significant effect on the prefrontal cortex-dependent working memory in mice.

关 键 词：工作记忆 β钙离子/钙调素依赖的蛋白激酶Ⅱ 前额叶 

分 类 号：Q6[生物学—生物物理学]