优势降酚菌的筛选及其生长条件优化研究  

Study on Screening of A Dominant Strain for Degradation of Phenol and Optimizing of Its Culture Conditions

作　　者：王祖佑 马庆霞[2] 陈怡 乔玮[2] 张忠智[2] 

机构地区：[1]兰州石化公司环保处,甘肃兰州730060 [2]中国石油大学(北京)化学科学与工程学院,北京102249

出　　处：《化学与生物工程》2010年第4期61-63,共3页Chemistry & Bioengineering

摘　　要：采用逐级驯化方法从活化污泥中筛选出可在1800 mg·L^-1苯酚浓度下生长的优势降解菌,命名为JF-6;并通过单因素实验对其生长条件进行了初步研究。结果表明,JF-6可在24 h内将1000 mg.L-1的苯酚完全降解,确定JF-6的最佳生长条件为：温度20-40℃、pH值4-9、NaCl浓度≤4.0%、外加氮源为蛋白胨、装液量40-100 mL·（250mL）^-1。The phenol-degrading strain named JF-6, which is screened from activation sludge, can grow in inorganic salt medium with 1800 mg ·L ^-1 phenol. The culture conditions are investigated by single factor experiment. The results show that the degradation rate of phenol reaches 100% at phenol concentration of 1000 mg. L 1. The optimum culture conditions are obtained as follows： temperature 20-40℃,pH value 4-9, NaCl concentration≤4.0%, DO （broth content） 40-100 mL of 250 mL shake flask,with peptone as additional nitrogen source.

关 键 词：苯酚 生长条件 生物降解 筛选 

分 类 号：Q939[生物学—微生物学]