壳聚糖多孔微球的制备与表征  

Preparation and characterization of porous chitosan microspheres

作　　者：李斌[1] 郑先创[2] 陈理兵[2] 葛海雄[2] 

机构地区：[1]东南大学附属中大医院口腔科,南京210009 [2]南京大学材料科学与工程系,南京210093

出　　处：《南京大学学报（自然科学版）》2010年第2期186-191,共6页Journal of Nanjing University（Natural Science）

基　　金：国家自然科学基金(50673040)

摘　　要：采用油包水包油(O/W/O)复相乳化悬浮交联的方法制备壳聚糖多孔微球,以低毒和无毒的乙酸乙酯和蓖麻油为制孔剂和非水溶性分散介质替代常用的具有一定毒性的有机分散剂甲苯和液体石蜡.乙酸乙酯先在壳聚糖醋酸溶液中乳化,然后上述乳液在蓖麻油中进一步反相乳化,采用戊二醛对壳聚糖分散相进行交联、固化.对制备工艺中影响壳聚糖多孔微球的多孔结构、粒径以及孔隙率等性能的影响因素进行了研究和优化.Chitosan, in particular with a format of microspheres, has been extensively employed in many applications, that is, treatment of wastewater, chromatographic support, enzyme immobilization, drug delivery, dental application, and hemostasis. In this paper, porous chitosan microspheres were prepared by oil-in-water-in-oil （O/W/O） multiple emulsion crosslinking method. The low toxic and nontoxic solvents, ethyl acetate and castor oil, were applied as a cavity generating agent and a wate^insoluble dispersion medium, respectively, to replace the commonly used toxic oil phase such as petroleum ether, toluene and paraffin. The low molecular weight chitosan was obtained by the depolymerization of raw chitosan with hydroperoxide. The viscosity molecular weight （Mw） of the chitosan was investigated by using an Ubbelohde viscosimeter and calculated based on Mark Houwink equation. For preparation of the porous chitosan microspheres, ethyl acetate was first emulsified in chitosan acetic acid solution, and subsequently the emulsion was inversely dispersed in castor oil. The chitosan micro-droplets were solidified and hardened by glutaraldehyde as crosslinking agent. All the preparation process was carried out at room temperature. The stirring rate of the emulsion, chitosan concentration, ethyl acetate/chitosan solution ratio, chitosan solution/castor oil ratio, and glutaraldehyde concentration were studied as effective preparation parameters on the size, size distribution and porosity of the chitosan microspheres. The chitosan mierospheres were filtrated and rinsed with ethanol and water to remove the organic solvent and the surfactants. Finally, the porous chitosan microspheres were obtained by freeze drying. The number average diameter of the optimized example of ehitosan mierospheres was 51.2 μm. The porous structures distributed in the chitosan microspheres were clearly observed by the SEM.

关 键 词：壳聚糖 多孔微球 乳化 制孔剂 

分 类 号：R318.0[医药卫生—生物医学工程] O636.1[医药卫生—基础医学]