LC-MS/MS同时测定左金丸和香连丸中6种生物碱的含量  

Simultaneous determination of 6 alkaloids in Zuojin Pill and Xianglian Pill by LC-MS/MS

作　　者：张新峰[1] 裘福荣[1] 蒋健[1] 高成璐[1] 贺敏[1] 

机构地区：[1]上海中医药大学附属曙光医院临床药理科,上海201203

出　　处：《中成药》2010年第4期597-600,共4页Chinese Traditional Patent Medicine

基　　金：高等学校博士学科点专项科研基金(200802680010);国家"重大新药创制"科技重大专项新药临床评价研究技术平台基金(GCP);中医肝肾疾病病证教育部重点实验室(上海中医药大学附属曙光医院)基金;上海市教委重点学科中药临床药理学基金(No.J50303);上海市申康医院发展中心临床药理学科研共享平台基金(No.SHDC12007202)

摘　　要：目的:建立左金丸(黄连、吴茱萸)和香连丸(黄连(吴茱萸制)、木香)中6种生物碱含量的LC-MS/MS测定方法。方法:色谱条件:Phenomenex Luna C18柱(5μm,250 mm×4.60 mm);乙腈-水(4 mmol/L乙酸铵和0.08%甲酸)为流动相,梯度洗脱;质谱条件:采用电喷雾离子源(ESI),正离子检测,MRM模式。结果:左金丸中药根碱、黄连碱、巴马汀、小檗碱、吴茱萸碱和吴茱萸次碱的平均回收率分别为101.0%,101.3%、101.2%、100.2%、99.6%、99.7%,RSD分别为1.1%、1.8%、0.98%、1.0%、1.3%、1.3%,香连丸中药根碱、黄连碱、巴马汀、小檗碱和吴茱萸碱的平均回收率分别为101.6%、99.8%、101.9%、101.0%、101.3%,RSD分别为1.0%、1.9%、0.75%、1.1%、1.9%。结论:本方法可同时测定左金丸和香连丸中药根碱、黄连碱、巴马汀、小檗碱、吴茱萸碱和吴茱萸次碱的含量,具有快速、简便等特点。AIM： To simultaneously determine contents of jatrorrhizine,coptisine,palmatine;berberinee,evodiamine and rutaecarpine in Zuojin Pill（Rhizoma Coptidis,Fructus Evodiae） Xianglian Pill（Rhizoma Coptidis（processed with Fructus Evodiae）,Radix Aucklandiae）.METHODS： Chromatographic separation was achieved with gradient elution by a C18 column hyphenated to an API 4000 mass spectrometer system was operated under the MRM mode with electrospray positive ionization（ESI）.The mobile phase A consisted of acetonitrile-water with 4 mmol/L ammonium acetate and 0.08% methanoic acid.RESULTS： The average recoveries of jatrorrhizine,coptisine,palmatine,berberinee,evodiamine and rutaecarpine in Zuojin Pill were 101.0%,101.3%,101.2%,100.3%,99.6%,99.7%,and the RSD were 1.1%,1.8%,0.98%,1.0%,1.3%,1.3%,respectively.The average recoveries of jatrorrhizine,coptisine,palmatine,berberinee and evodiamine in Xianglian Pill were 101.6%,99.8%,101.9%,101.0%,101.3%,and the RSD were 1.0%,1.9%,0.75%,1.1%,1.9%,respectively.CONCLUSION： This method is fast,sensitive and reproducible.It can be used to determine jatrorrhizine,coptisine,palmatine,berberinee,evodiamine and rutaecarpine in Zuojin Pill and Xianglian Pill under the same chromatogram condition.

关 键 词：左金丸 香连丸 药根碱 黄连碱 巴马汀 小檗碱 吴茱萸碱 吴茱萸次碱 LC-MS/MS 

分 类 号：R927.2[医药卫生—药学]