基于ITS序列分析铁皮石斛与近缘类群的亲缘关系  

Phylogenetic relationships of Dendrobium candidum and its related species based on DNA ITS sequences

作　　者：顾慧芬[1] 庄意丽[1] 马子建[2] 梅其春[2] 

机构地区：[1]上海市中药研究所,上海201203 [2]复旦大学生命科学学院,上海200433

出　　处：《中成药》2010年第4期628-632,共5页Chinese Traditional Patent Medicine

基　　金：上海市科委中药现代化专项项目(04Z19835-1)

摘　　要：目的:采用分子系统学方法分析铁皮石斛及其近缘种的遗传多样性,为准确进行基源鉴定、阐明属内的种间关系提供分子证据。方法:采集25种40个石斛样品分离提取DNA,PCR扩增ITS区及5.8S rDNA完整序列并测序,用Mega3.1软件进行分子遗传进化分析。采用邻接法和最大简约法分析铁皮石斛亲缘关系构造系统树。结果:对25种石斛类植物的ITS区序列进行分析,两种方法得到的系统树基本一致。ITS序列在石斛种间存在极其显著的差异,铁皮石斛与其它各种石斛均有各自特异性的序列位点。结论:ITS序列作为石斛属植物种间的分子鉴定标记是可行的。AIM： To apply molecular systematic techniques to revealing the genetic diversity of medicinal plant of Dendrobium candidum and its related species in Dendrobium.METHODS： The internal transcribed spacer（ITS） as well as 5.8S rDNA sequences of 40 samples of 25 species of Dendrobium were carried out on 25 dendrobi-um samples and amplified using PCR method and sequenced.Mega 3.1 was used to analyze the genetic diversity within the genus.Phylogenetic relationships among species in Dendrobium were estimated by maximum parsimony and neighbor-joining methods to construct similar ITS trees.RESULTS： The phylogenetic analysis indicated that Dendrobium candidum had a markedly difference in the interspecies and possessed the characteristic sequence site.CONCLUSION： ITS sequences as a good molecular marker for authentication between Dendrobium and outgroup is feasibility.

关 键 词：铁皮石斛 ITS序列 系统发育 亲缘关系 

分 类 号：R284.1[医药卫生—中药学]