甲基强的松龙对成骨细胞钙通道电流的影响  

The effect of methylprednisolone on the Ca^(2+) channel electric currents in osteoblasts

作　　者：贾丙申[1] 陈燕平[2] 高云[2] 张文杰[3] 薛延[4] 

机构地区：[1]海南医学院附属医院骨科,海南海口570102 [2]中国中医科学院望京医院,北京100102 [3]吉林大学生理学教研室,吉林长春130021 [4]北京积水潭医院骨科研究所,北京100035

出　　处：《海南医学院学报》2010年第4期403-406,428,共5页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(30472173)~~

摘　　要：目的:观察甲基强的松龙(mPSL)对MC3T3-E1成骨细胞L-钙通道电流(L-type voltage-sensi-tive calciumchannels current,L-VSCCsC)的影响。方法:设A组(正常对照组)、B组(10-6mol/L)、C组(10-5mol/L)和D组(10-4mol/L),各浓度的mPSL作用24h后,膜片钳技术记录每组细胞的L-VSCCsC(nA)。结果:正常对照组峰值电流为(-0.2284±0.0209,nA);10-6mol/L组为(-0.1991±0.0158,nA)下降12.8%;10-5mol/L和10-4mol/L组分别为(-0.1839±0.0179,nA)、(-0.1610±0.0033,nA)分别下降19.5%和29.5%,与正常对照组比较有显著性差异(P<0.01)。结论:mPSL剂量依赖性抑制成骨细胞L-VSCCsC。Objective：To observe the influence of methylprednisolone on the Ca^2＋ channel currents in osteoblasts.Methods：All were divided into four groups,A（control）,B（10-6mol/L）、C（10^-5mol/L）and D（10^-4mol/L）.The whole cell patch clamp technique was used in every MC3T3-E1 osteoblast of each group to test L-type voltage-sensitive calcium channels current（L-VSCCsC）.Results：The mean peak current of control group was（-0.2284±0.0209,nA）,B group was（-0.1991±0.0158,nA）with 12.8% decrease;C and D groups were（-0.1839±0.0179,nA）and（-0.1610±0.0033,nA）with 19.5% and 29.5% decrease,respectively.There was significant difference as compared with control（P0.01）.Conclusion：Methylprednisolone can inhibit the currents of L-calcium channels dose-dependently.

关 键 词：膜片钳技术 钙通道电流 甲基强的松龙 成骨细胞 

分 类 号：R336[医药卫生—人体生理学]