苜蓿内生菌中高效AHL降解菌的筛选和鉴定  

Screening and Identification of AHL-Degrading Bacteria from Alfalfa Entophytes

作　　者：汪玲玲[1] 龚伟伦[1] 刘霭莎[1] 

机构地区：[1]华南农业大学生命科学学院,广东广州510642

出　　处：《华南农业大学学报》2010年第2期68-71,共4页Journal of South China Agricultural University

基　　金：华南农业大学校长基金(4600-k07295);华南农业大学大学生科技创新活动(L07050)

摘　　要：从中国新疆、内蒙古和墨西哥等地分离苜蓿内生菌133株,平板筛选到8株细菌具有N-酰基高丝氨酸内酯(N-Acyl-L-homoserinelactone,AHL)降解活性,其中活性较强的菌株有r-12、r-9和R1.5.对8株AHL降解菌做AHL唯一碳源试验,结果发现菌株S35和N46在液体AHL唯一碳源培养基中生长情况较好,D600 nm分别为0.415和0.386,菌株r-9的生长情况最差,D600 nm为0.015.菌株R1.5还具有耐高温、耐盐碱、能分解无机磷、在无氮培养基上生长等优点.经细菌形态学观察、生理生化试验以及16S rDNA序列分析,初步确定菌株R1.5为1株地衣芽孢杆菌.One hundred and thirty-three alfalfa entophytes were isolated from the roots and stems of alfalfa grown in Xinjiang Province, Inner Mongolia Autonomous Region in China, and Mexico. Eight isolates showed N-Acyl-L-homoserinelaetone （AHL） degrading activity, especially strain r-12, r-9, RI. 5 showed stronger activity than others by the plate screening. When AHL used as unique carbon and energy, strain S35 （D600nm = 0. 386 ） and N46 （ D600nm = 0. 415 ） grew well, but D6oo of strain r-9 was 0. 015. Strain R1.5 grew well under high temperature, high salt concentration and high pH. Strain R1.5 also grew on the medium without additive nitrogen, and dissolved phosphor. Based on phenotype test, physi-biochemical results and 16S rDNA sequence analysis, strain R1.5 was identified as Bacillus licheniformis.

关 键 词：苜蓿内生菌 AHL降解菌 筛选 鉴定 

分 类 号：Q939.9[生物学—微生物学]