新加良附方影响人胃癌裸小鼠移植瘤新生血管形成的研究  

Studying the Effect of Modified Decoction of Liang-fu on Angiogenesis of Human Gastric Cancer in Nude Mice

作　　者：倪磊[1] 田劭丹[1] 马成杰[1] 孔焕宇[2] 陈信义[3,4] 

机构地区：[1]北京中医药大学东直门医院 [2]中国中医科学院望京医院 [3]教育部 [4]北京市重点实验室(中医内科学)

出　　处：《山西中医》2010年第4期50-52,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：高等学校博士点基金(新教师基金)资助(编号:200800261007)

摘　　要：目的:观察新加良附方对人胃癌裸小鼠移植瘤血管内皮生长因子(VEGF)表达和微血管密度(microvessel density,MVD)的影响,以探索其对人胃癌新生血管形成的作用。方法:建立移植型人胃癌MGC803实体瘤模型后,予模型对照及新加良附方大、中、小剂量4种处理并分组,分别取4组肿瘤组织,以免疫组化染色法检测肿瘤组织VEGF表达和以CD31为血管内皮标记的MVD。结果:与模型组比较,新加良附大、中剂量组有抑制MVD的作用,三者MVD值分别为68 880.66±35 846.10,37 386.44±20 175.69和40 531.48±20 817.34,后两者较前者有统计学意义(P<0.05)。且与模型组相比,新加良附大、中剂量组能够下调VEGF表达,其值分别为16 227.80±7 620.67,4 453.73±1 717.25和5 566.35±3 686.67,其差异有统计学意义(P<0.01)。结论:新加良附方能够抑制人胃癌新生血管形成。新加良附方抑制胃癌的作用可能与抑制胃癌新生血管形成有关。Objective： To observe the influence of Decoction of Liang-fu on vascular endothelial growth factor （VEGF） expression and microvessel density（MVD） in nude - mice -transplanted tumor of human gastric cancer, and investigate its effect on neovascularization of human gastric cancer. Method ： After building up solid tumor models MGC803 of transplanted type of human gastric cancer, we divided them into control group and treatment groups. Treatment group were divided into low, medium and high dose groups of Modified Decoction of Liang -fit. Respectively select tumor tissues in the four groups and detect VEGF and MVD marked by CD31 with immunohistochemical staining method. Result： Compared with model group, the medium and high dose groups of Modified Decoction of Liang -fit inhibited MVD. Three MVD values were 68 880.66 ±35 846.10, 37 386.44 ± 20 175.69 and 40 531.48 ±20 817.34, and the latter two was statistically significant than the former （P 〈0. 05）. And compared with model group, the expression of VEGF in medium and high dose groups of Modified Decoction of Liang -fit was lower than in control group, and the values were 16 227.80 ±7 620.67, 4 453.73 ±1 717.25 and 5 566.35 ±3 686.67 respectively. The difference was significant （P 〈0.01 ）. Conclusion： Modified Decoction of Liang -fu can inhibit angiogenesis of human gastric cancer. And its effect of inhibiting the growth of gastric cancer is related to inhibition of angiogenesis of human gastric cancer.

关 键 词：新加良附方 胃癌 血管内皮生长因子 微血管密度 实验研究 

分 类 号：R285.5[医药卫生—中药学]