微孔草Actin基因核心片段的克隆及序列分析  

Cloning and sequence analysis of Actin gene fragment of Microula sikkimensis

作　　者：吴淑娟[1] 张一弓[1] 张丽静[1] 傅华[1] 

机构地区：[1]农业部草地农业生态系统学重点开放实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2010年第4期97-101,共5页Pratacultural Science

基　　金：国家重点研究发展计划"973"(2007CB108903);国家自然科学基金(30800801);第二届草业科学研究生论坛;国家科技支撑计划(2008BADB3B08)

摘　　要：提取微孔草Microula sikkimensis叶片的总RNA,并以其为模板,运用RT-PCR方法扩增出Actin基因的核心序列。通过连接、转化后对阳性克隆进行PCR鉴定并测序,序列分析结果表明:微孔草Actin基因核心片段长599 bp,编码199个氨基酸。将该序列与GenBank中已注册的植物Actin基因序列进行同源性比较,发现它们之间的同源性在84%以上,氨基酸序列同源性在95%以上,具有高度的保守性。Total RNA of Microula sikkirnensis was extracted from leaf. The actin gene fragment was amplified by reverse transcription polymerase chain reaction RT-PCR and then cloned into pGEM-T vector. The gene fragment was sequenced after identifying the positive clone by PCR. The results revealed that the actin gene fragment from M. sikkirnensis contains 599 bp and encodes a protein of 199 amino acids. Similarity comparison with other actin gene sequence in the GenBank showed that it shared over 84 % of nucleotide sequence similarity and over 95 % of amino acid sequence similarity with other actin, which suggests that actin gene of M. sikkirnensis was highly conservative.

关 键 词：微孔草 ACTIN基因 序列分析 

分 类 号：Q78[生物学—分子生物学]