RB型硝胺发射药使用寿命实验研究  

Establishment of rapid test Brucella by PCR

作　　者：刘锴[1] 翟丽娟[1] 院富 王立伟 吴素云 LIU Kai,ZHAI Li-juan,YUAN Fu,WANG Li-wei, WU Su-yun （1.College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000,China; 2.Huade County Livestock Bureau,Huade 013350, China;3.Tongliao City Qianjiadian Vet Station,Tongliao 028000,China; 4.Tongliao City Kerchin District Disease Control Center,Tongliao 028000,China）

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]内蒙古化德县畜牧局,内蒙古化德013350 [3]通辽市科尔沁区钱家店兽医站,内蒙卉通辽028000 [4]通辽市科尔沁区疫控中心,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2010年第2期188-190,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：为了更准确地预测发射药的有效使用期,提出了以发射药力学性能变化的数据作为老化失效判据的观点。采用热加速老化试验的方法,研究了含黑索今双基药（RB型硝胺发射药）的抗冲、抗压强度、压缩率随老化时间和温度变化的规律。结果表明,该型发射药在在安全贮存期内,高分子材料老化降解造成的力学性能下降,不会影响其安全使用。RB型硝胺发射药的使用寿命远大于安全贮存寿命。A study was on the specificity and sensitivity about PCR of 31KDa protein gene for B.abortus. The DNA product is 223 bp. DNA were extracted with kit from 6 species Brucella. The DNA of 6 species Brucella strains were amplified by the primers of 31 KDa, but not for the DNA of Y. enterocolitica 0：9, E.coli 157 and S. typhimuium strains .A sensitivity of the PCR with examined Brucella DNA is 96fg. It was about 20 CFU.

关 键 词：材料学 RB硝胺发射药 使用寿命 贮存寿命 力学性能 热加速老化 

分 类 号：S852.61[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医]