Survivin与MMP-9在子宫内膜异位症在位内膜中的表达  

Expression of Survivin and MMP-9 in Eutopic Endometrium of Patients with Endometriosis

作　　者：米淑玲[1] 张军[2] 尹利荣[1] 马鸿达[3] 赵秀兰[3] 王宇全[1] 

机构地区：[1]天津医科大学第二医院妇科,300211 [2]山东省泰安市中心医院 [3]天津医科大学病理学教研室

出　　处：《天津医药》2010年第3期192-194,共3页Tianjin Medical Journal

摘　　要：目的:检测子宫内膜异位症(EMs)患者在位内膜中survivin与MMP-9的表达情况并分析两者表达的相关性。方法:收集80例子宫内膜异位症患者子宫内膜标本为EMs在位内膜组(EMs组),其中临床分期Ⅰ期4例、Ⅱ期25例、Ⅲ期44例与Ⅳ期7例;增殖期47例,分泌期33例。以20例同期手术的单纯子宫肌瘤患者子宫内膜作为对照组,其中增殖期内膜7例,分泌期13例。采用免疫组化二步法检测2组标本中survivin与MMP-9的表达;并应用IPP专业图像分析系统进行定量分析。结果:survivin与MMP-9均在子宫内膜胞浆中表达。在EMs组中增殖期survivin和MMP-9及分泌期survivin表达均高于对照组(P<0.01);其中,survivin表达与细胞周期无关,呈持续性高表达。EMs组不同临床分期间survivin表达,Ⅱ期明显高于Ⅲ期,其余各分期间表达差别无统计学意义(均P>0.05);而MMP-9在各AFS分期中表达差别均无统计学意义(均P>0.05)。Pearson相关性分析表明survivin与MMP-9在子宫内膜异位症在位内膜中表达呈正相关(r=0.262,t=2.860,P<0.05)。结论:survivin与MMP-9在子宫内膜异位症形成及进展过程中均起关键的作用。Objective： To investigate the expression of survivin and MMP-9 in the eutopic endometrium of patients with endometriosis （EMs）. Methods： Eighty patients with endometriosis were selected as EMs group, four of them were in clinical stageⅠ, 25 in stageⅡ, 44 in stage Ⅲ and 7 in stage Ⅳ, 47 in the proliferative phase and 33 in the secretory phase of the menstrual cycles. Twenty patients with hysteromyoma were selected as control group, including 7 patients in the proliferative phase and 13 in the secretory phase of the menstrual cycles. The expression levels of survivin and MMP-9 were determined by immuno-histochemical method. Image-Pro Plus 6.0 （IPP）, the special-purpose system for quantitative measurement of medical images, was used to analyse the results quantitatively. Results： Both survivin and MMP-9 were found positive in the plasma of the endometriosis. Both survivin and MMP-9 expression levels were higher in EMs group than those in the control group （P 0.01）. Besides, survivin was over expressed with no relation to the menstrual cycle of the endometium. Survivin expression was higher in endometriosis stage Ⅱ than that of the stage Ⅲ（P 0.05）. There was no difference in survivin expression within other stages（P 0.05）. While MMP-9 expression had no difference between all the endometriosis stages（P 0.05）. There was a close relationship between survivin and MMP-9 expression levels in 80 eutopic endometrium of endometriosis（r = 0.262, t = 2.860, P 0.05）. Conclusion： The over expression of survivin and MMP-9 may play an important role in the pathogenesis of en- dometriosis.

关 键 词：微管相关蛋白质类 基质金属蛋白酶9 子宫内膜异位症 子宫内膜 细胞凋亡 细胞外基质 

分 类 号：R711.71[医药卫生—妇产科学]