骨髓间充质干细胞移植促进脊髓神经功能修复机制的研究  

Research on the Effect of Bone Marrow Stroma Cell on Spine Cord Regeneration

作　　者：杨海云[1] 高忠礼[2] 朱本清[3] 

机构地区：[1]天津医院脊柱外科,300211 [2]吉林大学中日联谊医院骨科 [3]天津市人民医院骨科

出　　处：《天津医药》2010年第3期226-228,257,共4页Tianjin Medical Journal

摘　　要：目的:观察骨髓间充质干细胞(BMSC)移植对脊髓损伤后神经功能恢复、星形胶质细胞增生、空洞形成以及神经细胞凋亡的影响。方法:提取BMSC,制作SD大鼠脊髓损伤模型80只,随机分为对照组(A组)和细胞移植组(B组)。1周后再次手术,用微量注射器刺入B组脊髓损伤部位两端,分别缓慢推入10μLBMSC悬液,A组注入等量PBS,于移植后1、2、4、6、8周观察神经恢复情况;移植后第1、2、3、7、14天行TUNEL染色和电镜检查观察神经细胞凋亡情况。8周后行胶质纤维酸性蛋白(GFAP)及神经生长抑制因子Nogo-A免疫组化,测量各组脊髓空洞的大小。结果:8周后A组和B组神经功能评估(BBB评分)分别为(22.0±2.1)分和(28.5±2.7)分,14d时凋亡细胞数分别为14.4±2.7和9.1±3.3;白质中GFAP阳性细胞数分别为32.3±1.3和23.7±0.6,Nogo-A阳性神经元分别为(108.6±125.8)个和(78.3±12.4)个,空洞体积分别为(23.3±12.4)mm3和(14.5±10.6)mm3,2组间比较差别均有统计学意义(P<0.05)。结论:骨髓间充质干细胞可通过减少星形胶质细胞的增生,缩小空洞,抑制Nogo-A以及神经细胞凋亡等机制促进脊髓神经功能的恢复。Objective： To investigate the enhancing effect of bone marrow stroma cell （BMSC） on the functional recovery of injured spinal cord by observing the formation of gliotic scar, cavity volume and the cascade of apoptosis of neural cells. Methods： Eighty spinal cord injury （SCI）rat models were made and randomly divided into two groups： group A （n = 40）, the control group without any treatment; group B（n=40）, the injured animals treated with BMSC implantation. The behavioral evaluation was performed using Basso, Beattic and Bresnahan （BBB） scoring system. Scores were recorded at time points of 1, 2, 4, 6 and 8 weeks after transplantation. After 8 weeks, rats were sacrificed. The immunoreactivity of Nogo-A, glial fibrillary acidic protein （GFAP） and the cavity area were measured. The cell apoptosis was detected by TUNEL methods at 1, 2, 3, 7 and 14 days after injury. Results： Compared with control group, treated animals gained higher scores after 8 weeks of transplantation. The number and the size of reactive astrocytes, the average volume of cavity, TUNEL positive cells, the expression of Nogo-A and GFAP reduced significantly in group B compared with those of group A （P 0.05）. Conclusion： BMSC possess effects on repairing injured spinal cord and promoting functional recovery through various mechanisms.

关 键 词：骨髓细胞 间质干细胞 脊髓损伤 干细胞移植 模型 动物 大鼠 Sprague-Dawley 

分 类 号：R542.22[医药卫生—心血管疾病]