双酚A对人子宫内膜基质细胞增殖及雌/雄激素受体表达的影响  

Effects of bisphenol A on cell proliferation and expression of estrogen/androgen receptor in human endometrial stroma cells

作　　者：秦定霞[1] 李瑛[2] 刘晓慧[1] 钱晓乔[1] 蔡瑞芬[2] 崔毓桂[1] 刘嘉茵[1] 

机构地区：[1]南京医科大学第一附属医院临床生殖医学科,210029 [2]江苏省计划生育科学技术研究所

出　　处：《江苏医药》2010年第5期497-499,F0002,共4页Jiangsu Medical Journal

基　　金：国家教育部创新团队(IRT0631);江苏省科技厅项目(BM2008151)

摘　　要：目的观察双酚A对人子宫内膜基质细胞(hESCs)增殖和雌/雄激素受体(ERα/AR)表达的影响。方法体外培养hESCs,在6孔板贴壁达70%～80%后鉴定细胞纯度。采用无血清培养同步化,然后给予不同浓度的双酚A(0、10-8、10-6、10-4mol/L)处理48 h。Trizol裂解细胞提取总RNA,实时荧光定量PCR法测定基质细胞ERα和AR mRNA表达水平;采用流式细胞技术测定细胞周期。结果本文纯化培养方法得到的hESCs,波形蛋白染色阳性率达95%。10-6、10-4mol/L的双酚A对基质细胞增殖周期有抑制作用。10-4mol/L的双酚A使基质细胞ERαmRNA表达显著升高(P<0.05);10-6、10-4mol/L的双酚A也显著升高AR mRNA的表达水平(P<0.05);但是,10-8mol/L的双酚A则有抑制ERα/AR mRNA表达的趋势(P>0.05)。结论低剂量双酚A抑制人子宫内膜ERα/AR表达,而高浓度双酚A则升高ERα/AR表达,进而影响子宫内膜的功能。Objective To study the effects of bisphenol A on cell proliferation and expression of estrogen/androgen receptor（ERα/AR） in the human endometrial stromal cells（hESCs）.MethodshESCs were isolated and cultured in vitro and cell purity was identified when the cultural confluent condition reached 70%-80%.Cultured cells were treated with concentrations of bisphenol A（0,10-8,10-6 and 10-4mol/L） after synchronization by serum-free culture medium.Forty-eight hours after the treatment with bisphenol A,total RNA of the cultured stroma cells were extracted by Trizol and the mRNA expressions of ERα and AR were detected by real-time PCR.In addition,flow cytometry was used to detect the cell cycles.Results hESCs were successfully isolated and cultured in this study.Confirmed by vimentin immunochistochemical staining,cell purity was about 95%.Cell cycles were significantly inhibited by bisphenol A at the concentrations of 10-6 and 10-4 mol/L.The mRNA expression of ERα in cultured stroma cells was up-regulated by bisphenol A at the concentration of 10-4 mol/L（P0.05）.The expression of AR was significantly increased by bisphenol A at the concentrations of 10-6 and 10-4mol/L.However both ERα and AR mRNA were observed to have the trend to be inhibited by bisphenol A of 10-8 mol/L.Conclusion The expressions of ERα and AR in cultured hESCs are significantly inhibited by low concentration,but increased by high concentration of bisphenol A,by which bisphenol A influences endometrial functions.

关 键 词：双酚A 子宫内膜基质细胞 雌激素受体Α 雄激素受体 

分 类 号：R331[医药卫生—人体生理学]