糖基化终产物对小鼠成骨样细胞基质金属蛋白酶2分泌的影响  

Effect of advanced glycation end products on secretion of MMP-2 in cultured mouse embryo/fetus calvaria fibroblasts

作　　者：戴荣峰[1] 金晖[2] 孙子林[2] 董振华[1] 

机构地区：[1]常州市第三人民医院内分泌病科,213001 [2]东南大学附属中大医院内分泌病科

出　　处：《江苏医药》2010年第5期549-551,共3页Jiangsu Medical Journal

基　　金：常州市社会发展科技计划(CS2008219)

摘　　要：目的探讨糖基化终产物(AGEs)对小鼠成骨样细胞基质金属蛋白酶2(MMP-2)分泌的影响。方法用不同浓度的AGEs(50、100、200、400 mg/L)干预培养的小鼠成骨样细胞(MC3T3-E1)24 h和用200 mg/L的AGEs干预MC3T3-E1不同时间(12、24、48 h),以无血清培养基(α-MEM)和相应浓度的牛血清白蛋白(BSA)为对照。用Western blot法检测小鼠成骨样细胞上清中MMP-2蛋白的表达。结果不同浓度和不同时间的AGEs干预的MMP-2分泌水平明显高于对照组(P<0.01)。结论AGEs以时间及浓度依赖的方式增加小鼠成骨样细胞MMP-2分泌,AGEs可能通过调节成骨细胞MMP-2的分泌参与骨质疏松的发病。Objective To investigate the effects of advanced glycation end products（AGEs） on the secretion of matrix metalloproteinase-2（MMP-2） in cultured mouse embryo/fetus calvaria fibroblasts（MC3T3-E1）.Methods The cultured MC3T3-E1 was intervened with AGE-BSA（50,100,200,400 mg/L） for 24 h and with 200 mg/L AGE-BSA for 12,24,48 h,respectively,and α-MEM and BSA were taken as the negative control.The expression level of MMP-2 in the supernatant of MC3T3-E1 was detected by Western blot.Results The content of MMP-2 was significantly higher in the cells intervened with different concentrations AGE-BSA or with 200 mg/L AGE-BSA for different times（P0.01）.Conclusion The AGE-BSA can stimulate the secretion of MMP-2 in cultured MC3T3-E1 in a dose-and time-dependent manner.

关 键 词：糖基化终产物 成骨样细胞 基质金属蛋白酶2 

分 类 号：R336[医药卫生—人体生理学]