同种异体骨髓间充质干细胞移植上调急性心肌梗死大鼠Cx43的表达  

Transplantation of allogenic mesenchymal stem cells up-regulates connexin 43 expression in rats with myocardial infarction

作　　者：李金轶[1] 钟国强[1] 柯红红[1] 何燕[1] 温丽娜[1] 韦卓[1] 赵艳梅[1] 

机构地区：[1]广西医科大学第一附属医院广西心血管病研究所心内科,广西南宁530021

出　　处：《基础医学与临床》2010年第4期337-342,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30560051);广西留学回国人员科学基金(桂科回0448014);广西医疗卫生重点科研课题(桂卫重200301)

摘　　要：目的研究同种异体骨髓间充质干细胞(MSCs)移植心肌梗死(MI)大鼠后缝隙连接蛋白43(Cx43)在不同时期的动态变化。方法建立大鼠MI模型。将同种异体MSCs用5-氮胞苷诱导成心肌样细胞并行荧光标记,经二次开胸注射入MI大鼠梗死区和梗死边缘区。各亚组分别于移植后4、8和12周在荧光显微镜下跟踪MSCs移植情况。同时用免疫组化分析Cx43表达与缝隙连接(GJ)分布。结果MSCs体外诱导可分化为自发搏动的心肌样细胞,表达心肌特异性肌钙蛋白T(cTnT)和形成肌丝结构。MSCs移植后可长期存活并在4、8和12周,并有效上调缺血区Cx43的表达,改善GJ分布紊乱状态。在梗死区Cx43无特殊改变。结论MSCs具有分化为心肌样细胞的可塑性,移植后上调MI后缺血区Cx43表达,改善GJ分布紊乱。Objective To investigate the alterations of connexin 43（Cx43） expression and its distribution at different stages of myocardial infarction（MI） in rats after transplantation of allogenic mesenchymal stem cells（MSCs）.Methods Wistar rats were ligated on the left anterior descending coronary artery to make MI models.They were injected with allogenic MSCs,which were induced by 5-aza and labelled by DAPI,during the second operation after 7 days of MI.In subgroups,MSCs were detected by fluorescence microscope.Cx43 expression and GJ distribution were examined by immunohistochemistry after 4,8 or 12 weeks respectively.Results MSCs differentiated into cardiac muscle cell-like cells which were capable of pulsing spontaneously,expressing cTnT and forming myofilament in vitro.Transplanted MSCs can survive in MI host and upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.No change of Cx43 was seen at infarcted zones.ConclusionMSCs have the plasticity of differentiating into cardiac muscle cell-like cells which can continuously upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.

关 键 词：骨髓间充质干细胞 心肌梗死 缝隙连接蛋白43 

分 类 号：R542.22[医药卫生—心血管疾病]