子痫前期患者系统性氧化应激反应的检测  

Detection of systematic oxidative stress in preeclampsia

作　　者：钱中清[1,2] 曾耀英[1] 朱斌 季煜华[1] 何芳 

机构地区：[1]暨南大学组织移植与免疫实验中心,广东广州510632 [2]蚌埠医学院免疫学教研室,安徽蚌埠233030 [3]广州市何贤纪念医院产科,广东广州510632

出　　处：《基础医学与临床》2010年第4期343-347,共5页Basic and Clinical Medicine

基　　金：国家自然科学重点基金(30230305);国家自然科学青年基金(30500466)

摘　　要：目的研究子痫前期系统性氧化应激检测方法。方法组织化学染色检测胎盘组织缺氧表现;用胞内活性氧(ROS)探针H2DCFDA及全血染色方法检测中性粒细胞(PMN)胞内ROS水平;用H2O2检测试剂盒检测血清中H2O2水平。结果与正常妊娠胎盘组织比较,子痫前期患者胎盘组织合胞体细胞显著增多;血管破坏,结构不清,局部血管有纤维钙化特征;非孕妇女、正常妊娠妇女及子痫前期患者PMN胞内ROS水平分别为:45.61±12.20、51.02±13.60(P<0.01)、85.10±16.30(P<0.01);而血清H2O2浓度分别为:(24.57±5.17)μmol/L、(26.61±3.25)μmol/L、(39.84±9.67)μmol/L。结论对子痫前期系统性氧化应激进行定性和定量检测结果与疾病发生和发展一致。Objective To detect systematic oxidative stress in preeclampsia.Methods（1）Morphological features of placenta hypoxia were observed by histological method;（2）Level of granulocyte intracellular reactive oxygen species was monitored by dyeing full blood with 2′,7′-dichlorodihydrofluorescein diacetate（H2DCFDA）;（3）Level of H2O2 in sera was detected by special kits.Results Compared to normal pregnancy,placentas from preeclampsia showed distinct features of hypoxic stress injury,such as more syncytial knots formation,fibrosis emerged,vein injury and loss its normal configuration;Fluorescence values of ROS probe in neutrophils from different women were 45.61±12.20（n=49）,51.02±13.60（n=56,P〈0.01）and 85.10±16.30（n=47,P〈0.01）;Concentrations of H2O2 were（24.57±5.17）μmol/L（n=49）,（26.61±3.25）μmol/L（n=56,P〉0.01） and（39.84±9.67）μmol/L（n=47,P〈0.01） respectively.Conclusion With the help of histological method,flow cytometry and special kits,systematic oxidative stress can be detected through checking placentic tissues,netrophils and sera of preeclampsia.

关 键 词：子痫前期 氧化应激 胎盘 中性粒细胞 

分 类 号：R714.245[医药卫生—妇产科学]