移植胚鼠神经干细胞对大鼠脊髓损伤后红核神经元损伤的影响  

Effects of embryonic neural stem cells on trauma of red nucleus neurons of the rats with spinal cord injury after transplantion

作　　者：孔令胜[1] 聂冬丽[1] 张军臣[1] 张浩[1] 许华[1] 

机构地区：[1]济宁医学院附属医院神经外科,山东济宁272029

出　　处：《基础医学与临床》2010年第4期394-397,共4页Basic and Clinical Medicine

摘　　要：目的研究神经干细胞(NSCs)移植对大鼠脊髓损伤(SCI)后红核神经元的作用。方法5-溴脱氧尿嘧啶核苷(BrdU)法标记处于对数生长期的NSCs,采用电控脊髓损伤打击装置制作大鼠脊髓损伤模型。实验分为3组:NSCs组、SCI组和假手术组(Sham组)。SCI后3 d进行NSCs移植,用免疫组化法观察移植细胞的存活及迁移情况,用辣根过氧化物酶(HRP)逆行示踪技术标记红核神经元,并用四甲基联苯胺(TMB)呈色反应显示红核脊髓束神经元的存活情况,用行为学(BBB)评分法观察大鼠瘫痪肢体的恢复情况。结果在损伤脊髓区域可检测到BrdU标记的阳性NSCs,中脑HRP标记红核神经元数目明显多于SCI组(P<0.01),BBB评分亦明显高于SCI组(P<0.01)。结论体外培养的胚胎大鼠NSCs在移植到脊髓损伤区域后可存活和迁移,对SCI后中脑红核神经元具有保护作用,从而促进了大鼠肢体功能的恢复。Objective To study the effects of embryonic neural stem cells transplantion on trauma of red nucleus neurons of the rats with spinal cord injury.Methods NSCs in logarithmic phage were labeled with BrdU,a Sprague Dawley rat mode of spinal cord injury（SCI） was developed with electrocircuit control spinal cord injuring device.Thirty SD rats were randomly divided into three groups： sham group,SCI group and NSC group.The NSCs were transplanted into injured site three days after SCI.Then NSCs labeled with Brdu were detected by immunohistochemisty,rubrospinal tract（RST） neurons were labeled by retrograde transport of the horseradish peroxidase（HRP） from the lesion site,which were taken by damaged axons and remained in the neurons,then the labeled red nucleus（RN） neurons were counted.Hind limb function of experimental rats was evaluated by a blinder observer using BBB open field locomotion rating score.Results BrdU positive NSCs were detected in the spinal cord after transplantation.the number of RST neurons labeled by HRP in NSC group was more than that in SCI group（P〈0.01）,the BBB score of NSC group was higher than SCI group（P〈0.01）.Conclusion The transplanted NSCs can survive in the injured site of spinal cord and protect RN,then promote more remarkably functional recovery after SCI.

关 键 词：脊髓损伤 神经干细胞 红核神经元 大鼠 

分 类 号：R651.2[医药卫生—外科学]