8株牛支原体分离株P81表面膜蛋白基因的克隆与序列分析  

Cloning and sequence analysis of P81 gene of eight strains Myciokasna bovis in China

作　　者：董慧[1,2] 辛九庆[2] 李媛[2] 陈维[2] 刘洋[2] 张美晶[2] 姜海芳[2] 刘云[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第3期232-234,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：兽医生物技术国家重点实验室基本科研费(NKLVBP200814)

摘　　要：本研究对我国采集自8个省份患有牛呼吸道疾病的肺脏组织病料进行了病原分离鉴定,得到8株牛支原体(M.bovis)分离株。参考GenBank中已发表的M.bovisPG45株的P81基因序列,设计引物扩增P81基因,将其克隆到pMD-18T上,筛选重组质粒并对其进行序列测定及分析。结果显示,P81基因全长2097bp,含有1个开放性阅读框,编码699个氨基酸。这8株M.bovis分离株的P81同源性为99.4%～99.9%,与PG45株同源性94.6%～94.9%,与无乳支原体(M.agalacia)PG2株同源性仅为73.8%～74%,结果表明,M.bovisP81基因基因高度保守,种间差异较大,具有研究前景。Eight isolates of Myciokasna bovis were isolated from lung tissues afected cattle with respiratory disease from eight provinces. According to the reported nucleotide sequence of P81 gene of the Myciokasna bovis PG45 stain,a pair of primers was designed for amplification of the P81 gene. Then the amplified fragments were cloned into pMD-18T vector and sequenced. The results showed that the P81 gene was 2 097 bp in length,and included a complete open reading frame encoded a protein of 699 amino acids. The homology of nucleotide sequences for these eight isolates were 99.4 % to 99.9 % in P81 gene,94.6 % to 94.9 % with PG45 strain,and 73.8 % to 74% with M. agalacia PG2 strain. These results indicted that P81 gene of M. bovis were highly conserved,but difference between species,and the P81 gene is potential gene to be used in vaccine and diagnosis.

关 键 词：牛支原体 P81基因 序列分析 

分 类 号：S852.61[农业科学—基础兽医学]