哮喘大鼠淋巴液与血液CD4^+CD25^+Treg及IL-10、TGF-β水平比较和地塞米松干预的影响  

The comparison of CD4^+CD25^+Treg,IL-10 and TGF-β from lymph and blood in bronchial asthmatic rat and the effect of dexamethasone on it

作　　者：赵志旭[1] 冯学斌[1] 石涛[1] 杨成[2] 张立霞[2] 

机构地区：[1]滨州医学院儿科学教研室,山东滨州256603 [2]滨州医学院实验室中心,山东烟台264003

出　　处：《细胞与分子免疫学杂志》2010年第3期238-241,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省教育厅科技计划项目(J07YE14)

摘　　要：目的:观察CD4+CD25+Foxp3+调节性T细胞、IL-10、TGF-β在哮喘大鼠淋巴液与血液中的水平,以及地塞米松干预的影响,探讨其与哮喘发病的关系。方法:建立大鼠哮喘模型,收集激发后0、24、48 h淋巴液、血液中淋巴细胞,采用流式细胞术(FCM)检测淋巴液及血液中CD4+CD25+Foxp3+T细胞百分率;采用ELISA方法检测血浆及淋巴液中IL-10、TGF-β水平。结果:哮喘组淋巴液和血液的CD4+CD25+Foxp3+T细胞百分率水平、血浆IL-10和TGF-β浓度在各时间点均低于对照组和治疗组(P<0.05);哮喘组淋巴液中CD4+CD25+Foxp3+T细胞百分率水平、IL-10浓度在不同时间点均高于血液水平(P<0.05),而TGF-β浓度则低于血液水平(P<0.05);治疗组淋巴液和血液中CD4+CD25+Foxp3+T细胞百分率水平、血浆IL-10和TGF-β浓度与对照组相比无显著性差异(P>0.05)。结论:哮喘大鼠淋巴液中存在CD4+CD25+Foxp3+调节性T细胞数量以及功能失调,且淋巴液中CD4+CD25+Foxp3+调节性T细胞水平显著高于其血液水平,地塞米松可能通过影响CD4+CD25+Foxp3+调节性T细胞数量以及功能而发挥治疗作用。AIM：To observe the expression of CD4＋CD25＋Foxp3＋Treg,IL-10 and TGF-β in lymph and blood,as well as the effect of dexamethasone on it and investigate the relationship between them.METHODS：Lymph and blood samples of 0 h,24 h,48 h after the last challenge were collected from the rat model of asthma.The percentage of CD4＋CD25＋Foxp3＋Treg were detected by flow cytometer（FCM）,while the levels of IL-10 and TGF-β were determined by ELISA.RESULTS：The percentage of CD4＋CD25＋Foxp3＋Treg in the blood and the levels of IL-10 and TGF-β in the plasma in cases of asthma group were significantly lower than that of the control and therapy group at different time points（P0.05）;The percentage of CD4＋CD25＋Foxp3＋Treg and the level of IL-10 in lymph were significantly higher than that of blood（P0.05）,however,the level of TGF-β in lymph were significantly lower than that of blood（P0.05）;There were no significant differences which the percentage of CD4＋CD25＋Foxp3＋Treg in the blood and the levels of IL-10 and TGF-β in the plasma between control group and therapy group（P0.05）.CONCLUSION：There is a disbalance both quantity and function of CD4＋CD25＋Foxp3＋ in the lymph of bronchial asthmatic rat,and the percentage of CD4＋CD25＋Foxp3＋Treg in lymph are significantly higher than that of blood,which dexamethasone may effectively increase the percentage of CD4＋CD25＋Foxp3＋Treg in bronchial asthmatic rat.

关 键 词：哮喘 调节性T细胞 白细胞介素10 转化生长因子Β 

分 类 号：R562.2[医药卫生—呼吸系统]