清利生精丸对大肠埃希菌感染小鼠生殖细胞凋亡及Fas/FasL表达的影响  

Qingli Shengjing Pills decrease apoptosis of germ cells and expressions of Fas and FasL in male mice infected with Escherichia coli

作　　者：于爱莲[1] 邓文[1] 焦鹏[1] 申姜颖[1] 朱小明[1] 刘莉[1] 庄东明[1] 

机构地区：[1]泰山医学院病原生物学教研室,山东泰安271000

出　　处：《中华男科学杂志》2010年第3期280-284,共5页National Journal of Andrology

基　　金：山东省计生委资助项目(NO.ER08)~~

摘　　要：目的:探讨清利生精丸是否具有抗生殖细胞凋亡及影响Fas/FasL的表达作用,从分子水平进一步阐明清利生精丸治疗大肠埃希菌感染所致男性不育的机制。方法:将50只雄性昆明小鼠,经膀胱注射大肠埃希菌建立动物感染模型,观察15d后随机分为5组(10只/组):模型组(不治疗)、大剂量(22.5g/ml)、中剂量(13.5g/ml)、小剂量(4.50g/ml)3个清利生精丸治疗组、呋喃坦啶西药治疗组,编码依次为MN、MTa、MTb、MTc、MTd组;另取10只同样小鼠膀胱注射生理盐水作为正常对照组(编码为CT)。各治疗组连续灌胃药液10d后,应用流式细胞术检测小鼠睾丸生殖细胞凋亡率,免疫组化测定生殖细胞Fas/FasL蛋白表达水平和同时观察睾丸组织的病理变化。结果:MN组生殖细胞凋亡率(57.44%)与CT组(28.54%)、MTa组(30.11%)、MTb组(28.59%)相比,差异有显著性(P<0.01);MN组生殖细胞凋亡率与MTc组(46.54%)和MTd组(43.41%)比较,有所增加,但无显著性差异(P>0.05)。MN组小鼠睾丸生殖细胞Fas/FasL蛋白的表达水平明显高于CT、清利生精丸各治疗组和MTd组,差异有显著性(P<0.01);MN组小鼠睾丸组织出现明显的病理改变,其他各组未见明显异常。结论:实验雄性小鼠感染大肠埃希菌后,可导致小鼠睾丸生殖细胞凋亡增加,Fas/FasL蛋白的表达水平上升;清利生精丸通过降低Fas/FasL蛋白的表达,降低生殖细胞的凋亡率,提高生殖能力,可作为治疗大肠埃希菌感染性不育的有效药物之一。Objective：To study the effects of Qingli Shengjing Pills（QSP）on the apoptosis of germ cells and expressions of Fas and FasL in male mice infected with Escherichia coli（E.coli）,and to clarify the molecular mechanism of QSP in the treatment of male infertility induced by E.coli infection.Methods：Fifty male mice were injected with E.coli via the bladder to make infection models,and at 15 dpi equally randomized into five groups：an untreated,a high-dose QSP（22.5 g/ml）,a medium-dose QSP（13.5 g/ml）,a low-dose QSP（4.50 g/ml）and a Furadantin treatment group,which were coded as MN,MTa,MTb,MTc and MTd,respectively.Another 10 mice were injected with saline and included in the control group coded as CT.After 10 days of oral medication,the apoptosis of germ cells in the testis of the mice was detected by flow cytometry,the expressions of Fas and FasL determined by immunohistochemistry and the histopathological changes observed simultaneously.Results：After the treatment,the apoptosis of germ cells was observed in all the infected groups,and the apoptosis level in MN（57.44 %）was significantly higher than that in CT（28.54 %）,MTb（28.59 %）or MTa（30.11 %）（P 0.01）but had no significant difference from that in MTc（46.54 %）or MTd（43.41%）（P 0.05）.The expressions of Fas and FasL proteins were significantly higher in MN than in CT,MTa,MTb,MTc and MTd（P 0.01）.Histopathological changes of the testis tissue were observed in MN,but not in other groups.Conclusion：E.coli infection could increase the apoptosis rate of germ cells and the expressions of Fas and FasL proteins.Qingli Shengjing Pills,capable of enhancing reproductivity by reducing the expressions of Fas and FasL and apoptosis of germ cells,can be used as one of the effective drugs for infertility induced by E.coli infection.

关 键 词：清利生精丸 大肠埃希菌 动物模型 感染 生殖细胞凋亡 不育 雄性小鼠 

分 类 号：R285.5[医药卫生—中药学]