槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响  

Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration

作　　者：严梅娣 叶孟[2] 林蕾[2] 倪曙民[2] 王少敏[2] 刘素荣[2] 

机构地区：[1]宁波市第七医院普外科 [2]宁波大学医学院附属医院

出　　处：《中国临床药理学与治疗学》2010年第2期126-129,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省自然基金项目(Y206243);宁波市博士基金项目(2005A610020);宁波市医学科研项目(200530)

摘　　要：目的:探讨小鼠腹腔内注射槲寄生凝集素对腹腔巨噬细胞功能的影响。方法:16只小鼠分为腹腔内给药组和空白组,分别取腹腔内巨噬细胞并计数,采用ELISA法测定TNF-α表达情况,Griess法测定NO表达,MTT法检测腹腔内巨噬细胞对肠癌HCT116细胞的杀伤作用。结果:腹腔内给槲寄生凝集素后可明显增加腹腔内巨噬细胞数,并增加TNF-α和NO的表达水平;激活的巨噬细胞对HCT116细胞的杀伤作用增强,其杀伤作用与TNF-α和NO表达时间一致。结论:腹腔内注射槲寄生凝集素可激活腹腔内巨噬细胞,激活的巨噬细胞对HCT116细胞有杀伤作用。AIM： To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS： 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS： It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-α and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION： Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro.

关 键 词：结直肠肿瘤 槲寄生凝集素 巨噬细胞 腹腔内化疗 

分 类 号：R965.2[医药卫生—药理学]