西洛他唑对小鼠慢性缺血性脑损伤的保护作用及机制  

Protective effects of cilostazol on the chronic ischemic brain injury in mice and its action mechanism

作　　者：叶夷露[1] 张琦[1] 陈丽萍[2] 张建亭[1] 张晓华[1] 俞月萍[1] 魏尔清[3] 

机构地区：[1]浙江医学高等专科学校 [2]浙江衢州学院医学系 [3]浙江大学医学院

出　　处：《中国临床药理学与治疗学》2010年第2期130-134,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省卫生厅科研项目(2008B034);浙江医学高等专科学校自然科学基金(2006xz01)

摘　　要：目的:观察西洛他唑对小鼠慢性缺血性脑损伤的保护作用,探讨其与促血管生成的关系。方法:以大脑中动脉栓塞方法诱导小鼠局灶性脑缺血,缺血后1、4、7 h和术后1～14 d腹腔注射西洛他唑(10 mg/kg),每天一次,观察缺血后35 d西洛他唑对神经症状评分、斜板角度、脑梗死体积、神经元密度和缺血侧血管内皮生长因子(VEGF)、血管内皮生长因子受体2(Flk-1)表达的作用。结果:西洛他唑能降低缺血后神经症状评分,提高斜板角度,减少脑梗死体积,增加存活神经元密度和VEGF、Flk-1表达的数目。结论:西洛他唑对小鼠慢性局灶性脑缺血具有保护作用,其作用机制可能与诱导缺血侧VEGF、Flk-1表达,促进血管生成有关。AIM： To observe the protective effects of cilostazol on chronic ischemic brain injury in mice and the relationship to angiogene- sis. METHODS： Focal cerebral ischemia was induced by middle cerebral artery occlusion （MCAO）. Cilostazol （10 mg/kg） was i. p. injected at 1, 4, 7 h and 1-14 d once a day after ischemia. The neurological deficits, angle in inclined board test, infarct volume, neuron density, and the expressions of VEGF and Flk-1 in ischemic region were determined at the 35 d after ischemia. RESULTS： Cilostazol （10 mg/kg） significantly reduced the neurological deficits and infarct volume, and increased the angle in inclined board test, the neuron density, and the expressions of VEGF and Flk-1. CONCLUSION： Cilostazol has neuroprotective effect on chronic ischemic brain injury in mice, which may relate to angiogenesis via inducing the expressions of VEGF and Flk-1.

关 键 词：西洛他唑 血管内皮生长因子(VEGF) 血管内皮生长因子受体2(Flk—1) 脑缺血 

分 类 号：R965.2[医药卫生—药理学]