全自动免疫组化染色仪与手工免疫染色的比较  

Comparison of the automatic immunohistochemistry stainer and the manual immunostaining

作　　者：余琦[1] 李宁[1] 杨江辉[1] 周会芹[1] 刘成龙[1] 罗焕超[1] 

机构地区：[1]解放军第309医院病理科,北京100091

出　　处：《局解手术学杂志》2010年第2期83-85,共3页Journal of Regional Anatomy and Operative Surgery

摘　　要：目的观察比较Ventana全自动免疫组化染色仪与手工免疫染色的应用。方法选用常规应用的多种免疫试剂一抗和同种二抗,并采用机器操作与手工操作两种方法进行免疫组化染色,对比实验结果。结果应用全自动染色仪阳性染色着色均匀,定位准确,无边缘效应,背景清晰,各项指标均优于手工操作染色的组织。结论使用全自动免疫组化染色仪不仅标准化程度高,利于规范操作,更减少了手工操作染色带来的人为误差,能为临床病理诊断提供更准确的依据。Objective To observe and compare the application between Ventana automatic immunohistochemistry stainer and the manual immunostaining.Methods Several kinds of primary antibodies which were applied to the routine work and the same secondary antibodies were stained by the machine and manual immunohistochemistry operation respectively,and were compared.Results The active results stained by automatic immunohistochemistry stainer of which every index was prior to the manual operation were shown to the well-distributed coloration,exact location,non-edge-effect and clear background.Conclusion The automatic immunohistochemistry stainer is not only high-degree standardizaion and good to normative operation,but also reduces the personal error in manual staining.It could provide exact evidence for clinical pathological diagnosis.

关 键 词：全自动免疫组化染色仪 免疫组织化学 病理诊断 

分 类 号：R446.62[医药卫生—诊断学]