Accumulation of 1-deoxynojirimycin in silkworm,Bombyx mori L.  

作　　者：Hao YIN Xin-qin SHI Bo SUN Jing-jing YE Zu-an DUAN Xiao-ling ZHOU Wei-zheng CUI Xiao-feng WU 

机构地区：[1]Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Taian 2 71018, China [2]Institute of Sericultural Sciences of Siehuan Province, Nanchong 637000, China [3]College of Animal Science, Zhejiang University, Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2010年第4期286-291,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

摘　　要：1-deoxynojirimycin (1-DNJ) contents in the silkworm,Bombyx mori,at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood,digestive juice,and alimentary canal,but no 1-DNJ was observed in the silkgland. Moreover,1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar,while excreted at later stages of larval development. Further,it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.1-deoxynojirimycin （1-DNJ） contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.

关 键 词：SILKWORM 1-deoxynojirimycin （1-DNJ） ACCUMULATION Bombyx mori 

分 类 号：Q966[生物学—昆虫学]