吗啡对体外培养的人正常输卵管黏膜上皮细胞内MOR mRNA表达的影响  

MORPHINE-INDUCED REGULATION OF μ-OPIOID RECEPTORS IN HUMAN OVIDUCTAL EPITHELIAL CELL

作　　者：武晓丽[1] 郭述真[1] 

机构地区：[1]山西医科大学第一临床医学院妇产科,太原030001

出　　处：《中国药物依赖性杂志》2010年第2期97-100,共4页Chinese Journal of Drug Dependence

基　　金：山西省科技厅科研攻关基金资助项目(002047)

摘　　要：目的:研究吗啡对体外培养的人输卵管粘膜上皮细胞的μ阿片受体信使核糖核酸(MOR mRNA)表达的影响。方法:体外分离培养人输卵管粘膜上皮细胞,并通过免疫细胞化学的方法进行鉴定;将细胞分为对照组、吗啡组(10-4、10-5、10-6、10-7mol.L-1),通过RT-PCR的方法半定量分析吗啡对人输卵管粘膜上皮细胞MOR mRNA表达的影响。结果:吗啡组人输卵管粘膜上皮细胞中的MOR mRNA表达较对照组显著下降(P<0.05),且与吗啡浓度负相关(r=-0.966,P<0.05)。结论:外源性阿片类药物吗啡抑制人输卵管粘膜上皮细胞μ阿片受体mRNA的表达。Objective：To explore the effects of morphine on μ-opioid receptor mRNA expression in human oviductal epithelial cells.Methods：Human oviductal epithelial cells were isolated and cultured in vitro.These cells were identified by microscopy and immunocytochemical staining.All cells were randomly divided into control group and morphine groups（10-4,10-5,10-6,10-7 mol·L-1）.The effect of morphine on the MOR mRNA expression in human oviductal epithelial cells was observed by RT-PCR technique.Results：The expression of μ-opioid receptor mRNA in morphine groups was significantly lower than that in control group（P0.05）,and it was negatively correlated with morphine（r=-0.966,P0.05）.Conclusion：Exogenous opiate like morphine could inhibit the expression of MOR mRNA in human oviductal epithelial cells.

关 键 词：人输卵管粘膜上皮细胞 细胞培养 Μ型阿片受体 吗啡 

分 类 号：R691.3[医药卫生—泌尿科学]