山茱萸炮制前后石油醚部位化学成分及生物活性研究  

Studies on Chemistry Component and the Biological Activity of Petroleum Ether Extraction from Pre-and Post-processed of Cornus officinalis

作　　者：李冠业[1] 姚运香[1] 丁霞[1] 

机构地区：[1]南京农业大学化学系,江苏南京210095

出　　处：《中药材》2010年第2期192-195,共4页Journal of Chinese Medicinal Materials

基　　金：国家"十五"科技攻关课题(2001BA701A11)

摘　　要：目的:比较山茱萸炮制前后石油醚部位化学成分及对小鼠免疫功能的影响,探讨山茱萸炮制增效的物质基础。方法:采用GC-MS分析石油醚部位挥发性成分。以小鼠碳粒廓清指数K、吞噬指数α、脾脏和胸腺指数评价非特异性免疫功能。以小鼠血清溶血素水平评价特异性体液免疫功能。结果:山茱萸炮制前后石油醚部位化学成分发生明显变化,炮制后维生素E提高了46.6%,亚油酸提高了18.3%,亚麻酸甲酯提高了30.9%。山茱萸炮制后石油醚部位显著增强小鼠非特异性免疫功能和特异性体液免疫功能。结论:山茱萸石油醚部位是提高机体免疫功能的活性部位,炮制后其作用增强。物质基础可能是维生素E、亚油酸和亚麻酸甲酯等。Objective：To investigate substance basis for improving pharmacodynamics by comparing the chemical constituents of petroleum ether extraction from crude and processed Cornus officinals and the effects on the immunologic function of mice with immunosuppression induced. Methods： The volatile components in petroleum ether extraction were analyses by GC-MS. Non-specific immune function was determined by cleaning carbon particle index K, Swallow index α, spleen index and thymsu index. The specific humoral immune function was evaluated by detecting the content of serum hemolysin.Results： The chemical constituents of petroleum ether extraction of Cornus officinalis before and after being processed had significant changes. After being processed, Vitamin E increased by 46.6%, linoleic acid increased by 18.3% and methyl linen increased by 30.9%. The extraction increased clearance rate of charcoal carbon particles index K, swallow index α,spleen index, thymus index and the level of serum hemolysin . Conclusion： The extraction can markedly improve non-spcific immune function and the specific humoral immune function which are active sites of improving immunoloigc function and post-processed is better. Substance basis could be Vitamin E, Linoleic acid, methyl linen and so on.

关 键 词：山茱萸 炮制 石油醚部位 化学分析 免疫功能 

分 类 号：R283.1[医药卫生—中药学]