HPLC指纹图谱在白花丹提取工艺对比中的应用  

Process Comparison of Fingerprints of Different Extracts of Plumbago zeylanica by High Performance Liquid Chromatography

作　　者：胡佳惠[1] 高莉[1] 彭晓明[1] 霍仕霞[1] 闫明 

机构地区：[1]石河子大学药学院,新疆石河子832003 [2]新疆维吾尔自治区维吾尔医药研究所,新疆乌鲁木齐830049

出　　处：《中药材》2010年第2期272-275,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划项目(2007BAI48B06);区科研院所改革与发展专项基金(2009032)

摘　　要：目的:建立并优化白花丹的最佳色谱条件,采用HPLC指纹图谱选择白花丹药材最佳的提取方法。方法:以保留时间、相对峰面积为指标,综合评判,对白花丹半仿生提取法、醇提取法与水提取法进行工艺对比。结果:建立了白花丹最优的HPLC-FPs分离条件,3种提取工艺得到4个共有峰,白花丹醇提物与半仿生提取物的指纹图谱相关性较好,得到9个共有峰。白花丹药材用半仿生提取优于水提和醇提。结论:白花丹半仿生提取有望取代水提、醇提法,为其入血成分、制剂工艺及药效学研究奠定了基础。Objective：To optimize the separation conditions of fingerprints of Plumbago zeylanica by high performance liquid chromatography, on this basis, to choose the best way for extracting Plumbago zeylanica by high performance liquid chromatography. Methods： The investigation of HPLC-FPs of the different extraction samples was by the retention time and the relative area of common peaks in fingerprints. Results： We established the optimum separation conditions of HPLC-FPs for Plumbago zeylanica and there were 4 common peaks in fingerprints. According to the contrast research, the HPLC-FPs of the 90% ethanol extraction and SBE extraction of Plumbago zeylanica were quite similar, and they both had 9 common seaks in fingerprints, It was better to use SBE rather than WE in the extraction of Plumbago zeylanica. Conclusion： The SBE may replace the WE and ethanol extraction of Plumbago zeylanica.

关 键 词：白花丹 高效液相色谱 指纹图谱 工艺对比 

分 类 号：R284.2[医药卫生—中药学]