基于生物信息学分析方法设计HSV-1诊断芯片的通用寡核苷酸探针  

The Design of Oligonucleotide Probe in Herpes simplex virus type 1 Based on Bioinformatics

作　　者：崔智威[1] 曾照芳[1] 申崇标[1] 

机构地区：[1]重庆医科大学检验系,临床检验诊断学教育部重点实验室,重庆400016

出　　处：《激光杂志》2010年第2期63-64,共2页Laser Journal

摘　　要：目的：设计单纯疱疹病毒1型（HSV-1）诊断芯片的寡核苷酸（Oligo）探针;方法：利用BLAST软件对HSV-1的基因序列进行对比,得出对设计HSV-1探针有意义的特异序列,并通过生物学软件Oligo 6.0设计特异性高Tm值相近、长度均一的Oligo探针;结果：获得在相同的杂交、清洗条件下可得到最佳杂交效果的10条30-mer的Oligo探针;结论：通过生物信息学方法设计出HSV-1诊断芯片的探针,可为后期制备成基因芯片,为进行HSV-1的检测打下良好的基础。Objective：To design oligonucleotide probes for microarray detection of HSV-1 virus.Methods：By analyzing the DNA of HSV-1 with BLAST program,a group of specific sequences for the candidate probes was acquired.Oligo6.0 software was applied to analyze the candidates to select the probes with high specificity,identical length and similar melting temperature（Tm）.Results：Best 10 oligonucleotide probes of hybridization results were designed in the same hybridization and washing conditions.Conclusion：Oligonucleotide probes can be designed with BLAST program and Oligo6.0 software,and become a good foundation for preparing on DNA chips as the microarray for HSV-1 detection.

关 键 词：单纯疱疹病毒1型 基因芯片 BLAST Oligo探针 

分 类 号：R477[医药卫生—护理学]