雷帕霉素对人脐静脉内皮细胞增殖的影响  

Effect of rapamycin on proliferated human umbilical vein endothelial cell

作　　者：管丽丽[1] 吴伟[2] 李平华[3] 刘晓熹[4] 鄢秀菊[4] 

机构地区：[1]重庆医科大学附属第一医院核医学科,重庆400016 [2]重庆市公安局渝中区分局物证鉴定所,重庆400013 [3]重庆医科大学附属第一医院眼科,重庆400016 [4]重庆市第一人民医院眼科,重庆400013

出　　处：《激光杂志》2010年第2期75-76,共2页Laser Journal

摘　　要：目的:观察雷帕霉素对人脐静脉内皮细胞增殖的影响,探讨其可能的抑制新生血管的作用机制。方法:采用四唑盐比色试验(MTT)法检测不同浓度(0.1ng/ml,1ng/ml,10ng/ml,20ng/ml)的雷帕霉素对血管内皮生长因子(Vascular endothelial growth factor,VEGF)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)增殖的抑制作用。结果:不同浓度的雷帕霉素均能抑制由VEGF诱导的HUVEC增殖。结论:雷帕霉素能抑制HUVEC的增殖,且呈剂量依赖。雷帕霉素通过抑制血管内皮细胞的增殖来抑制新生血管的形成。Objective：To abserve the effect of rapamycin on the proliferation of human umbilical vein endothelial celI（HUVEC）in vitro.Methods：HUVEC were exposed to different concentrations of rapamycin （0.1ng/ml-20ng/ml） solution,and the influence of rapamycin on cell proliferation induced by VEGF was investigated by MTT assaying method.Results：Various concentrations rapamycin inhibited cell proliferation.Conclusion：rapamycin had the concentration dependent inhibitory effect in HUVEC.The mechanism of rapamycin on neovascularization is to inhibit the proliferation of HUVEC.

关 键 词：雷帕霉素 人脐静脉内皮细胞 新生血管 

分 类 号：TN248.1[电子电信—物理电子学]