奶牛NUCB2基因突变及其与产奶量关联的分析  

Analysis of Mutation of the NUCB2 Gene and Its Association with Milk Production in Dairy Cow

作　　者：刘俊霞[1] 李飞[1] 陈宏[2] 雷初朝[2] 任刚[2] 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]西北农林科技大学动物科技学院,杨凌712100

出　　处：《中国奶牛》2010年第4期10-11,共2页China Dairy Cattle

基　　金：国家863计划项目(No.2006AA10Z197);国家自然科学基金(No.30771544);国家科技支撑计划(2006BAD01A10-5)

摘　　要：利用PCR-SSCP和DNA测序技术首次研究了中国荷斯坦牛NUCB2基因的变异。结果表明在NUCB2基因外显子9发现两个沉默突变:27451G>A和27472T>C,两个碱基变化没有导致氨基酸的改变,但两个位点沉默突变同时发生,表现出3个基因型,即MM、MN和NN,经过对不同基因型与奶牛产奶量性状的关联分析,发现这些突变对奶牛产奶量产生显著影响(P<0.05)。The mutations of NUCB2 gene were studied with PCR-SSCP and DNA sequencing methods in Chinese Holstein. The results showed that two mutant sites with g27451G〉A and g27472T〉C were detected only at the exon9 of NUCB2 gene. These two silent mutation did not result in the change of amino acid, but they happened simutaneously. They showed three genotypes, MM, MN and NN. The different genotypes of these two mutation loci were significantly associated with milk production （P〈0.05）.

关 键 词：NUCB2基因 PCR—SSCP 单核苷酸多态性(SNPs) 产奶量 

分 类 号：S823[农业科学—畜牧学]