麦白霉素及麦迪霉素的活性测定比较  

Comparison of Determination of Meleumycin and Midecamycin by Turbidimetric Method

作　　者：高燕霞[1] 姜建国[1] 韩彬[1] 王茉莉[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《中国药事》2010年第4期347-350,共4页Chinese Pharmaceutical Affairs

基　　金：2006年河北省科技厅课题(编号06276445)

摘　　要：目的建立浊度法测定麦白霉素及麦迪霉素的活性的方法,并对其进行评价比较。方法以金黄色葡萄球菌为试验菌,加菌量为2.0%-2.5%（V/V）,37℃±0.5℃培养4-5h左右测定。结果麦白霉素抗生素线性浓度为1.0-3.0IU·mL^-1,麦迪霉素抗生素线性浓度为0.5-2.5IU·mL^-1。结论本方法灵敏,快速,影响因素较少,可作为测定评价麦白霉素及麦迪霉素效价的方法。Objective To establish a turbidimetric method for determination of meleumycin and midecamycin.Methods The test organism was Staphylococcus aureus and cultured with fresh medium（pH 7.0）.Adjust spore suspension of Staphylococcus aureus inoculate with 2.0%-2.5%（V/V）ratio,at 37℃±0.5℃ for about 4-5h.Results The linear range of acetyl meleumycin was 1.0-3.0IU·mL^-1,and that of Midecamycin was 0.5-2.5IU·mL^-1.Conclusion The method is sensitive,rapid and feasible to determine meleumycin and midecamycin.

关 键 词：活性 浊度法 麦白霉素 麦迪霉素 

分 类 号：R927.32[医药卫生—药学]