HPLC法测定坤宁颗粒中盐酸水苏碱的含量  

Determination of Stachydrine Hydrochloride in Kunning Granules by HPLC

作　　者：郑倩[1] 毕雪艳 李晓敏[2] 

机构地区：[1]河北省石家庄市中医院,石家庄050051 [2]邯郸市药品检验所

出　　处：《中国药事》2010年第4期392-393,共2页Chinese Pharmaceutical Affairs

摘　　要：目的采用HPLC法测定坤宁颗粒中盐酸水苏碱的含量。方法以氨基柱为色谱柱,以乙腈-水(78∶22)为流动相,以202nm为检测波长,流速为1.0mL.min-1。结果盐酸水苏碱在本文设计的范围内线性关系良好,r为0.9999,平均回收率为98.75%。结论该质量标准的研究有效控制了坤宁颗粒的质量,方法简单、结果准确、灵敏度高。Objective To establish a HPLC method for determination of stachydrine hydrochloride in Kunning Granules.Methods It was determined on an amido column,using acetonitrile-water（78∶22）as mobile phase at flow rate of 1.0mL·min^-1,and detected at 202nm.Results The linear relation of the peak area of stachydrine hydrochloride was determined by HPLC with a good regression coefficient,r=0.9999,and average recovery rate was 98.75%.Conclusion It can be used for controlling the quality of Kunning Granules.The method is simple,accurate and sensitive.

关 键 词：坤宁颗粒 盐酸水苏碱 HPLC 

分 类 号：R288[医药卫生—中药学] R927.2[医药卫生—中医学]