头发中烟碱和可替宁的超高效液相色谱测定  

Determination of Nicotine and Cotinine in Human Hair by UPLC

作　　者：刘正聪[1,2] 陆舍铭 刘春波[1,3] 招云芳[1,2] 王海利[1,3] 温亚东 缪明明 

机构地区：[1]红塔烟草集团有限责任公司技术中心 [2]云南大学化学科学与工程学院,昆明市翠湖北路2号650091 [3]云南民族大学化学与生物技术学院,昆明市一二一大街134号650031

出　　处：《烟草科技》2010年第3期38-41,共4页Tobacco Science & Technology

摘　　要：采用5%NaOH水溶液超声萃取-超高效液相色谱法同时测定了36个主动吸烟者头发样品和10个被动吸烟者头发样品中的烟碱和可替宁含量。结果表明:①烟碱和可替宁的RSD分别为3.52%和2.99%;回收率均为98.5%～101.6%;②主动吸烟者和被动吸烟者头发样品中均检测到烟碱,而可替宁只在主动吸烟者头发样品中检出;③主动吸烟者头发中的烟碱含量的高低主要是由其烟龄和日均吸烟支数决定的,而与其生理年龄关系较小;④主动吸烟者头发中的可替宁含量高低主要是由其烟龄决定的,而与其日均吸烟支数和生理年龄关系较小。该法操作简便,准确性高,重复性好,可用于批量头发样品中烟碱和可替宁含量的快速分析。The hair samples of 36 smokers and 10 passive smokers were extracted with 5% sodium hydroxide water solution under ultrasonic and the contents of nicotine and cotinine were determined by ultra performance liquid chromatography （ UPLC）. The results indicated that： 1 ） the recoveries of nicotine and cotinine were from 98.5% to 101.6% with RSDs of 3.52% and 2.99% , respectively; 2） nicotine was detected in all samples, while cotinine was detectable only in samples of smokers; 3） the nicotine content in hair mainly affected by smokers＇ smoking age and average number of cigarettes smoked daily rather than their physiological ages; 4） the content of cotinine in hair mainly depended on smoker＇ s smoking age, while slightly correlated with average number of cigarettes smoked daily or smoker＇ s physiological age. This method is simple, accurate, repeatable and can be used for rapid analysis of nicotine and cotinine in batches of hair samples.

关 键 词：超高效液相色谱 烟碱 可替宁 头发 

分 类 号：TS416[农业科学—烟草工业]