HPLC法同时测定鞘蕊苏中三种劳丹烷型二萜类成分  

Simultaneous HPLC Determination of 3 Labdane Diterpenoids in Coleus forskohlin (wild) Briq.

作　　者：张伟[1] 许云龙[2] 金歧端[2] 

机构地区：[1]昆明制药集团股份有限公司药物研究院,云南昆明650100 [2]中国科学院昆明植物研究所,云南昆明650204

出　　处：《云南中医学院学报》2010年第2期4-7,共4页Journal of Yunnan University of Traditional Chinese Medicine

摘　　要：目的:建立HPLC法测定鞘蕊苏药材中异佛司可林、1-乙酰基佛司可林、1,6-二乙酰基佛司可林的含量。方法:采用luna C8柱(250mm×4.6mm),以乙腈-水梯度洗脱,流速为1.0mL/min,紫外检测波长为210nm,进样量20μL,采集时间40min。结果:异佛司可林、1-乙酰基佛司可林、1,6-二乙酰基佛司可林的线性范围分别为1.0～16.0μg(r=0.9998),0.9～7.2μg(r=0.9999),7.5～60μg(r=0.9998).平均回收率(n=9)分别为97.8%,95.1%,97.6%.结论:本方法简便、准确,可为评价不同产地的鞘蕊苏药材中异佛司可林、1-乙酰基佛司可林、1,6-二乙酰基佛司可林的含量提供依据。Objective： To establish a HPLC method for determination of three constituents： including isoforskolin, 1 -actylforskolin and 1, 6 -diactylforskolin in Coleus forskohlin （wild） Briq. Methods： The reversed phase HPLC system consisted of a lunaCs column （250mm ×4. 6mm, 5μm）, The mobile phase consisted of acetonitrile （A） and H20 （B）. At a flow rate of 1. 0 mL/min, the gradient elutions were 50A： 50B to 35A ：65B in 30 min, 35A： 65B to 50A： 50B in next 10 min, 50A： 50B to 50A： 50B in last 5 min. The detection wavelength was 210 nm, the injection volume was 20μL and the column temperature was maintained at 25℃. Resuits： The Labdane Diterpenoids were successfully separated. The linear ranges of the Labdane Diterpenoids were 1.0 - 16.01μg （r =0. 9998）, 0. 9 -7.2μg （r =0. 9999）, 7.5 -60μg （r =0. 9998） respectively; The average recoveries of Labdane Diterpenoids were 97.8%, 85.1%, 97.6% respectively. Conclusion： The contents of the Labdane Diterpenoids in root of Coleus forskohlin （wild） Briq collected from different regions were determined. This method is rapid and accurate.

关 键 词：鞘蕊苏 劳丹烷型二萜 异佛司可林 1-乙酰基佛司可林 1 6-二乙酰基佛司可林 高效液 相色谱法 

分 类 号：R284.1[医药卫生—中药学]