RP-HPLC法同时测定芫花药材中7种黄酮类成分的含量  

RP-HPLC simultaneous determination of seven flavonoids in Flos Genkwa

作　　者：逄楠楠[1] 毕开顺[1] 闫宝庆[1] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2010年第4期633-636,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立RP-HPLC法同时测定芫花药材中芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素含量。方法:采用Kromasil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.05%磷酸水溶液梯度洗脱,流速1.0 mL·min^(-1);检测波长为338 nm,柱温40℃。结果:在上述条件下,芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素的质量浓度分别在1.21～12.1μg·mL^(-1)(r=0.9995),0.797～7.97μg·mL^(-1)(r=0.9997),0.320～3.20μg·mL^(-1)(r=0.9993),1.01～10.1μg·mL^(-1)(r=0.9997),2.02～20.2μg·mL^(-1)(r=0.9999),1.13～11.3μg·mL^(-1)(r=0.9996),1.92～19.2μg·mL^(-1)(r=0.9999)范围内与色谱峰面积呈良好的线性关系,低、中、高浓度的平均加样回收率(n=3)均在96.0%～100.8%,RSD均小于2.8%。结论:该分析方法简便、快速、准确,重现性好,为更好地控制芫花药材的质量提供方法。Objective：To establish a method for the determination of genkwainin -5 -O -β-D - primeveroside, genkwanin -5 -O -β-D -glucoside,luteolin,tiliroside,apigenin,hydroxygenkwanin and genkwanin in Flos Genkwa simultaneously.Methods：The separation was performed on a Kromasil C18 column（250 mm×4.6 mm,5μm） with a gradient elution composed of methanol and 0.05%phosphate acid.The column temperature was set at 40℃with flow rate of 1.0 mL·min^-1.The detective wavelength was set at 338 nm.Results：The linear range was 1.21 -12.1μg·mL^-1（r =0.9995）,0.797 -7.97μg·mL^-1（r =0.9997）,0.320 -3.20μg·mL^-1（r =0.9993）,1.01 -10.1μg·mL^-1（r =0.9997）,2.02-20.2μg·mL^-1（r =0.9999）,1.13 - 11.3μg·mL^-1（r =0.9996）,1.92 - 19.2μg·mL^-1（r = 0.9999） respectively（n = 6）.The average recoveries（n = 3） of seven flavonoids were 96.0%-100.8% with RSDs of the recoveries（n =9） were less than 2.8%.Conclusion：The assay demonstrated that the method is simple,rapid,accurate and reproducible for the quality control of Flos Genkwa.

关 键 词：芫花 RP-HPLC 黄酮类化合物 同时含量测定 

分 类 号：R917[医药卫生—药物分析学]