紫外波长切换高效液相色谱法同时测定壮骨关节丸中7种有效成分的含量  

HPLC simultaneous determination of seven active compounds in Zhuanggu Guanjie pills with switching UV wavelength

作　　者：颜冬梅[1] 王跃飞[1] 刘亚男[1] 谭沛 高秀梅[1] 

机构地区：[1]天津中医药大学中医药研究院,现代中药发现与制剂技术教育部工程研究中心,天津300193 [2]三九医药股份有限公司,深圳518029

出　　处：《药物分析杂志》2010年第4期637-640,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：天津市科技创新专项资金项目(编号07FDZDSH01011)资助;国家科技支撑计划项目(编号2007BAI47B01)资助

摘　　要：目的:采用紫外波长切换高效液相色谱法,建立同时测定壮骨关节丸中7种有效成分含量的方法。方法:色谱柱ZorbaxSB-C_(18)(4.6 mm×250 mm,5μm);流动相0.05%甲酸水溶液-乙腈,梯度洗脱;切换时间和检测波长:0～25 min,283 nm;25～30 min,365 nm;30～32 min,270 nm;32～37 min,246 nm;37～65 min,322 nm;柱温:40℃;流速:1.0 mL·min^(-1)。结果:柚皮苷、槲皮素、淫羊藿苷、补骨脂素、异补骨脂素、芒柄花素和蛇床子素在相应的浓度范围内与峰面积呈良好线性关系,相关系数均大于0.9997;平均回收率(n=6)在97.8%～104.3%之间,RSD均小于4.0%。结论:本方法简便快速、准确、精密度高、重复性好,适用于壮骨关节丸的质量控制。Objective：To establish a method for simultaneous determination of seven active components in Zhuanggu Guanjie pills using switching UV wavelength.Method：The HPLC analysis performed on a Zorbax SB - C18column with the mobile phase composed of acetonitrile and 0.05%formic acid water in gradient elution.Detection wavelength was set at 283 nm in 0 - 25 min,365 nm in 25 - 30 min,270 nm in 30 - 32 min,246 nm in 32 - 37 min,322 nm in 37 -65 min.The column temperature was maintained at 40℃.The flow rate was fixed at 1.0 mL·min^-1.Results：Naringin,quercetin,icariin,psoralen,isopsoralen,formononetin and osthole showed good linearity over the tested ranges（r≥0.9997）.The average recoveries were ranged from 97.8 to 104.3%with RSDs≤4.0%.Conclusion：The developed method was used for quantification of seven active components in Zhuanggu Guanjie pills,which had advantage of accuracy,repeatability and simplicity.

关 键 词：紫外波长切换 高效液相色谱法 壮骨关节丸 

分 类 号：R917[医药卫生—药物分析学]