抗肺纤胶囊对肺纤维化大鼠肺组织NF-_κ BmRNA、ICAM-1、TGF-β_1的影响  

Effect of Kang-fei-qian Aapsule on Bleomycin-induced Pulmonary Interstitial Fibrosis Rats about Expression of NF-_κ βmRNA,ICAM-1 and TGF-β_1

作　　者：陈云凤[1] 扈晓宇[2] 高堂玲[1] 

机构地区：[1]成都市中西医结合医院,四川成都610041 [2]成都中医药大学附属医院

出　　处：《成都中医药大学学报》2010年第1期53-56,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家中医药管理局资助课题(编号:04-05LP41)

摘　　要：目的:探讨具有补益肺肾、化瘀通络功效的抗肺纤胶囊抗肺纤维化的作用机制。方法:建立BLM诱导的大鼠肺间质纤维化动物模型,将实验动物分为6组:假手术组,模型对照组,抗肺纤胶囊高、中、低剂量组,强的松(阳性对照药)组,在第28天处死动物,应用原位杂交检测核转录因子κB(NF-κB)mRNA在大鼠肺组织中的表达,采用免疫组化技术测定细胞间粘附分子1(ICAM-1)、转化生长因子β1(TCF-β1)在大鼠肺组织中的表达,结合显微图象分析,对BLM大鼠肺组织中NF-κBmRNA的表达及ICAM-1、TGF-β1的表达进行了定量研究。结果:抗肺纤胶囊能明显抑制BLM大鼠肺组织中NF-κBmRNA、ICAM-1、TGF-β1的高表达(P<0.05,0.01),并呈现出一定的剂量依赖关系。结论:具有补益肺肾、活血通络作用的抗肺纤胶囊可阻断肺泡炎继续发展的中心环节——NF-ΚB的活化,抑制ICAM-1、TGF-β1等促炎及促肺纤维化细胞因子的表达,从而调控从肺泡炎到肺纤维化的病理进程,是其抗肺纤维化的一个重要环节。Objective:To evaluate the curative effect of KangFeiQian capsule on the rats model of bleomycin-induced pulmonary interstitial fibrosis and its mechanism.Methods:BLM-induced pulmonary fibrosis in rat animal model of experimental animals were divided into 6 groups: pseudo surgery,model control group,KangFeiQian capsule high,medium and low-dose group,prednisone(positive control drug) group.The rats were killed after 28 days of treatment.In situ hybridization detection of Nuclear factor-κB(NF-κB) mRNA in rat lung tissue.By immunohistochemistry determination of intercellular adhesion molecule-1(ICAM-1),transforming growth factor-β1(TGF-β1) in the rat lung tissues.The combination of micro-image analysis,the bleomycin-induced pulmonary fibrosis lung tissue expression of NF-κBmRNA and ICAM-1,TGF-β1 expression carried out a quantitative study.Results:KangFeiQian capsule could inhibit the high expression of NF-κBmRNA,ICAM-1,TGF-β1 in the bleomycin-induced pulmonary fibrosis of rat lung tissue(P<0.05,0.01),and has shown a certain dose-dependent relationship.Conclusion:The results suggested that it is effective to use KangFeiQian capsule in treatment pulmonary interstitial fibrosis;the treatment of tonify lung and kidney,resolve blood stasis and dredge collateral is an effectual therapy of Traditional Chinese Medicine(TCM).KangFeiQian capsule has some protective effects on pulmonary interstitial fibrosis in rats,partly through decreasing NF-ΚB,ICMA-1,TGF-β1 expression in the lung.

关 键 词：肺间质纤维化 中医药疗法 抗肺纤胶囊 实验研究 NF-κβmRNA ICAM-1 TGF-β1 

分 类 号：R285.5[医药卫生—中药学] R563[医药卫生—中医学]