邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯联合染毒对雄性大鼠生殖毒性的影响  

Combined Toxic Effects of Di-n-butyl Phthalate and Di-2-ethylhexyl Phthalate on Fertility in Male Rats

作　　者：田晓梅[1,2,3] 李玲[1,2,3] 宋琦如 刘桂珠[1,2,3] 张鹏举[1,2,3] 董桂清[1,2,3] 吴生俊 刘静环[1,2,3] 王现知 

机构地区：[1]宁夏医科大学公共卫生学院环境卫生学教研室 [2]宁夏生殖与遗传重点实验室 [3]省部共建生育力保持教育部实验室,宁夏银川750004

出　　处：《环境与健康杂志》2010年第4期290-292,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30760208)

摘　　要：目的探讨邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)和邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)联合染毒对雄性大鼠睾丸的脂质过氧化作用以及对生化酶和血清激素水平的影响。方法选择健康成年清洁级雄性SD大鼠32只,按2×2析因设计随机分为4组,分别为阴性对照组(玉米油)、DBP单独染毒组(1/20LD50,1.0g/kg)、DEHP单独染毒组(1/20LD50,1.7g/kg)和DBP+DEHP染毒组(1.0g/kgDBP+1.7g/kgDEHP),每组8只。采用经口灌胃方式进行染毒,染毒剂量为5ml/kg,每天1次,连续染毒8周。测定睾丸组织中超氧化物歧化酶(SOD)、还原性谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、γ-谷氨酰转肽酶(γ-GT)水平,测定血清中雄性激素睾酮(T),黄体生成素(LH)、促卵泡激素(FSH)水平。结果 DBP和DEHP联合染毒对睾丸组织中SOD活力和血清中T水平呈现协同作用,对γ-GT、ACP活力和FSH水平呈现拮抗作用。结论 DBP和DEHP联合染毒对雄性大鼠具有明显的生殖毒性作用,可能是通过破坏氧化-抗氧化系统的平衡和影响睾丸组织中酶的活力以及血清激素水平干扰下丘脑—垂体—睾丸轴的生理平衡,抑制T的分泌,导致雄性生殖功能的降低。Objective To study the combined toxic effects of di-n-butyl phthalate （DBP）and di-2-ethylhexyl phthalate （DEHP） on sex hormone and lipid peroxidation in male rats. Methods According to 2×2 factorial analysis, thirty-two healthy and clean adult male SD rats were randomly divided into 4 groups,including a control group （given coin oil） and three experimental groups： DBP（1/20 LD50, 1.0 g/kg, dissolved in coin oil）, DEHP （1/20 LD50, 1.7 g/kg, dissolved in coin oil） and DBP＋DEHP（1.0 g/kg ＋ 1.7 g/kg, dissolved in coin oil）, 8 rats in each group, through garage, once a day, for 8 consecutive weeks. The spectrophotometric method was used to measure the activity of lipid peroxidation SOD, GSH and GSH-Px level in testis homogenate. The activities of ACP, AKP and γ-GT were assessed in testis homogenate. Radioimmunoassay was used to determine the testosterone, LH and FSH levels in the serum. Results There was synergism between DBP and DEHP on the SOD activity in testicle and testosterone level in serum, and there was antagonism between DBP and DEHP on the γ-GT and ACP activity in testicle, as well as the FSH level in serum. Conclusion DBP combined with DEHP can cause obvious toxic effects on reproductive function in male rats. The change of testosterone biosynthetic enzymes and the levels of T in serum and disordered physiologic balances of hypothalamic-pituitarytestis axis may be key factors contributing to the decrease of testosterone, then the decrease of reproductive function in male rats.

关 键 词：生育力 邻苯二甲酸二丁酯 邻苯二甲酸二(2-乙基己基)酯 睾酮 

分 类 号：R994.6[医药卫生—毒理学]