NF-κ B激活在兔急性心肌梗死再灌注后无复流中的意义  

Effect of Nuclear Factor-κ B on No-reflow Phenomenon after Reperfusion of Acute Myocardial Infarction in Rabbits Model

作　　者：吕媛[1] 陈韵岱[2] 骆景光[1] 杨秀秀[1] 田峰[1] 辛毅[1] 金泽宁[1] 吕树铮[1] 

机构地区：[1]首都医科大学附属北京安贞医院心内科,北京100029 [2]中国人民解放军总医院心内科,北京100853

出　　处：《现代生物医学进展》2010年第4期601-605,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30772289)

摘　　要：目的:观察心肌核因子-κB(NF-κB)在急性心肌梗死(AMI)再灌注后无复流的活化情况,探讨NF-κB促进无复流发生发展的作用机制。方法:24只新西兰大白兔随机分为假手术组(冠状动脉只穿线不结扎)和缺血再灌注组(结扎冠状动脉2小时,再灌注1小时),每组12只。采用凝胶阻滞迁移分析方法(EMSA)检测正常区、缺血区和无复流区心肌组织中NF-κB活性;ELISA法测定不同时点血浆中白细胞介素-6(IL-6)、超敏C反应蛋白(CRP)以及肿瘤坏死因子-α(TNF-α)的含量;光镜、电镜观察心肌组织病理变化。结果:(1)与正常区相比,缺血区和无复流区心肌组织中NF-κB活性异常升高(P<0.01)。(2)与结扎前相比结扎后2h、再灌注后1h血浆IL-6、CRP、TNF-α水平呈进行性升高(P均<0.05)。(3)NF-κB的活性与无复流面积、血浆IL-6、CRP以及TNF-α水平呈正相关(分别为r=0.844,P<0.01;r=0.682,P<0.05;r=0.687,P<0.05;r=0.893,P<0.01)。(4)无复流面积与血浆IL-6、CRP以及TNF-α水平呈正相关(分别为r=0.861,P<0.01;r=0.806,P<0.01;r=0.877,P<0.01)。(5)光镜及电镜结果显示无复流区的心肌组织损伤较缺血区更为严重。结论:急性心肌梗死再灌注后无复流现象的发生可能与局部心肌组织中NF-κB的过度活化有关,活化的NF-κB通过促进IL-6、TNF-α等炎症因子的表达,参与无复流的发生发展过程。Objective： To investigate myocardial nuclear factor-κB （NF-κB） activation and expression of Interleukin-6（IL-6）, C-reactive protein（CRP） and Tumor necrosis factor-alpha（TNF-α） on no-reflow phenomenon during postischemiac reperfusion. Method： A total of 24 New Zealand White rabbits were divided randomly into the sham-operated group （n=12） and ischemicreperfusion group （n=12）. Anesthetized rabbits underwent 2h of coronary occlusion followed by 1h reperfusion. The activity of NF-κB in normal area, area at risk of infarction（AR） and no-reflow area（NR） were evaluated by EMSA. Artery blood of both groups were obtained at 5min before ligation,2h after ligation,1h after reperfusion. Plasma concentrations of IL-6, CRP and TNF-α were measured by ELISA. Observation pathological changes of myocardial tissue. Results： （1）Comparing with normal area and AR, activity of NF-κB in no-reflow area was significantly increased （P〈0.01）.（2） Plasma concentrations of IL-6, CRP and TNF-αwere significantly increased during the reperfusion period （respectively, P〈0.05）. （3）The area of no-reflow and plasma levels of IL-6, CRP and TNF-αwere positively correlated with activity of NF-κB（respectively, r=0.844,P〈0.01; r=0.682, P〈0.05; r=0.687, P〈0.05; r=0.893,P〈0.01）.（4） Plasma levels of IL-6, CRP and TNF-αwere positively correlated with the area of no-reflow （respectively, r=0.861,P〈0.01; r=0.806, P〈0.01; r=0.877, P〈0.01）. （5） Pathological changes of no-reflow area was more serious than normal area and AR. Conclusion： The no-reflow phenomenon after AMI might attribute to myocardial NF-κB activation.

关 键 词：心肌梗死 无复流 炎症 NF-ΚB 

分 类 号：Q95-3[生物学—动物学] R542.22[医药卫生—心血管疾病]