超氧化物歧化酶和Bcl-2在实验性精索静脉曲张大鼠睾丸中的变化(英文)  

The Variation of SOD and Bcl-2 in the Testes of Experimental Varicocele Rats

作　　者：贾月峰[1] 王新生[1] 马征[1] 王沛涛[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266021

出　　处：《现代生物医学进展》2010年第4期620-624,F0002,共6页Progress in Modern Biomedicine

摘　　要：目的:建立大鼠实验性精索静脉曲张(experimental varicocele EV)的模式,测量睾丸中超氧化物歧化酶(superoxide dismutaseSOD)活性和Bcl-2的表达。方法:将40只雄性青春期Wistar大鼠随机分为EV8周组和12周组(各12只)和相应的假手术对照组2组(各8只),通过部分结扎左肾静脉建立大鼠EV模型,分别于术后8周、12周处死动物,测左侧精索静脉直径,用比色法测SOD 活力,免疫组化法测Bcl-2的表达。结果:成功建立了EV型,与相应的对照组相比左侧精索静脉直径明显增大(P<0.01)。光学显微镜下观察睾丸组织,发现大鼠睾丸生精上皮退变,曲细精管萎缩,间质水肿和精子发育阻滞。EV组双侧睾丸的SOD活性显著低于相应的对照组(P<0.01),左侧睾丸比右侧睾丸更低,但无明显统计学意义(P>0.05)。EV组双侧睾丸间质细胞中Bcl-2的染色指数与相应的对照组相比均显著降低(P<0.01),左侧睾丸染色指数比右侧睾丸下降更明显(P<0.01),EV12周组与 EV8周组相比,EV12周组染色指数更低(P<0.05)。SOD活性与Bcl-2的染色指数在0.01水平有显著相关性。结论:超氧化物歧化酶通过抑制氧化应激可能影响Bcl-2并抑制细胞凋亡。Objective： To establish experimental varicocele （EV） model in rats to measure the superoxide dismutase （SOD） activity and the Bcl-2 expression. Methods： The varicocele model was built by partial ligation of the left renal vein in adolescent male Wistar rats. 40 male adolescent Wistar rats were randomly divided into 8-week EV group, 12-week EV group and the corresponding sham-operated control group. The rats were killed 8 weeks and 12 weeks after operation, and the activity of SOD was determined by Colorimetry and the Bcl-2 was determined by Immunohistochemistry. Results： The experimental varicocele model was successfully builded. Compared with the corresponding control group, the diameter of the left spermatic vein enlarged remarkably （P〈0.001）. When observed the testicular histology under optical microscope, it was found that degeneration in germinal epithelium,seminiferous tubule atrophy, interstitial edema and spermatogenesis retardation in the testes of EV rats. The SOD activity of the bilateral testes in EV groups decreased significantly compared with that in the corresponding control groups （P〈0.01） and the left sides were lower than the right sides, but had no statistical significance （P〈0.05）. The staining indices of Bcl-2 in bilateral testicular mesenchymal cells of the EV groups decreased significantly compared with that in the corresponding control group （P〈0.01）, and the left sides decreased more significantly than the right sides （P〈0. 01）, the staining indices decreased more significantly in EV12 group than that in EV8 group （P〈0.05）. The correlation between the activity of SOD and the staining indices of Bcl-2 is significant at the 0.01 level. Conclusion： SOD may have effect on Bcl-2 and inhibit apoptosis by inhibiting the oxidative stress.

关 键 词：精索静脉曲张 氧化应激 凋亡 超氧化物歧化酶 BCL-2 

分 类 号：Q95-3[生物学—动物学] R697.24[医药卫生—泌尿科学]