腹部肠管火器伤后肺组织坏死因子-α和核因子-κB表达水平的变化研究  

Expression of TNF-α and NF-κ B in the lung after intestinal perforations due to abdominal firearm wound

作　　者：胡良煜[1] 刘江伟[2] 高伟[2] 

机构地区：[1]兰州军区乌鲁木齐总医院检验科,新疆乌鲁木齐830000 [2]兰州军区乌鲁木齐总医院肝胆外科,新疆乌鲁木齐830000

出　　处：《现代生物医学进展》2010年第4期652-654,共3页Progress in Modern Biomedicine

基　　金：兰州军区医药卫生科研基金资助项目(LXH-2005019)

摘　　要：目的:探讨腹部肠管火器伤后肺脏组织肿瘤坏死因子-α(TNF-α)、核因子-κB(NF-κB)的动态变化规律。方法:健康长白仔猪42头随机等分为对照组和腹部肠管火器伤后1h、2h、4h、8h、12h和24h实验组。用免疫组化图像分析法测定各组肺组织TNF-α和NF-κB表达水平,在光镜下观察各组肺脏组织学变化。结果:伤后各组肺组织TNF-α和NF-κB表达水平明显高于对照组,二者于伤后1h至8h内快速上升并,在8h出现高峰(P<0.05)。伤后各组逐渐出现肺泡腔变窄,肺泡壁增厚;肺充血,肺间质水肿。结论:TNF-α、NF-κB参与了腹部肠管火器伤后机体的病理生理过程,可能是引起腹部肠管火器伤后多器官功能障碍(MODS)的重要因素之一。Objective： To investigate the changes of pulmonary TNF-αand NF-κB expression levels and their dynamic changing regularity in lung injury after intestinal perforations due to abdominal firearm wound. Methods： A total of 42 Chang-Bai piglets were randomized into 7 groups：control group and wounded 1hour group, 2hour group, 4hour group, 8hour group, 12hour group, 24hour group. The model of intestinal perforations due to abdominal firearm wound was established in wounded groups. Pulmonary TNF-αand NF-κB expression levels were measured with immunohistochemical staining and image analysis in all groups. The alterations of pulmonary tissue were observed under light microscope in all groups. Results： Levels of pulmonary TNF-αand NF-κB expression in wounded groups were significantly elevated compared to control group, and from 1h to 8h rapidly upgrade, they appeared one peak in 8h group group （P 0.05）. The experimental group of 4 h,8h, 12h, 24h appeared gradually aggravated alveolar space narrowing and alveolar wall thickening, pulmonary congestion, interstitial edema. Conclusion： TNF-α and NF-κB participated in the pathophysiological process after intestinal firearm wound, may be one of the important causes leading to MODS after intestinal firearm wound.

关 键 词：腹部损伤 火器伤 肿瘤坏死因子-α 核因子-ΚB 

分 类 号：R654.2[医药卫生—外科学]