气相色谱法测定行军散中丁香酚的含量  

Assaying of Eugenol in Xingjunsan by Gas Chromatography Method

作　　者：王喆桅[1,2] 金城[1] 焦懿[1,3] 王伽伯[1] 朱珊薇[1] 谭睿[2] 肖小河[1] 

机构地区：[1]解放军302医院中药研究所,北京100039 [2]西南交通大学生命科学与工程学院,四川成都610031 [3]湖南中医药大学药学院,湖南长沙410007

出　　处：《解放军药学学报》2010年第2期137-138,共2页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：重大新药创制专项;No.2009ZXJ09004-057

摘　　要：目的建立行军散中丁香酚的含量测定方法。方法色谱柱为HP-5(25m×0.32mm×0.52μm);固定相为5%苯基甲基聚硅氧烷;FID检测器;进样口温度240℃;起始柱温110℃;程序升温;载气为氩气。结果在该色谱条件下,提取物挥发油中丁香酚的含量在65.9～395.4μg范围内呈良好线性关系(r2=0.9991)。结论该方法具有便捷、准确、重复性高等优点,可以作为行军散中丁香酚含量的测定方法。Objective To determine the content of eugenol in Xingjunsan by gas chromatography Method. Methods The gas chromatography colum was HP-5 （25 m×0.32 mm×0.52 μm）,and the static phase was 5% phenylmethylpolysiloxane.With the FID detector, the temperature of sample injection was 240℃ while the initiation temperature was 110 ℃.The programmed heating method was used with argon as carrier gas. Results The linearity of eugenol content was good within the range of 65.9-395.4 μg（r2=0.9991）. Conclusion This method is simple, accurate and reproducible, and can be used for assaying of eugenol in Xingjunsan.

关 键 词：行军散 丁香酚 气相色谱法 

分 类 号：R927.2[医药卫生—药学]