拟南芥抗菌核病突变体275-7的初步研究  

Preliminary Studies on Sclerotinia sclerotiorum Resistant Mutant 275-7 of Arabidopsis thaliana

作　　者：李丹[1,2] 黄军艳[2] 张学江[2] 刘学群[1] 覃瑞[1] 刘胜毅[2] 

机构地区：[1]中南民族大学生命科学院,武汉430074 [2]中国农业科学院油料作物研究所,中国农业部油料作物生物学重点开放实验室,武汉430062

出　　处：《中国农业科技导报》2010年第2期81-85,共5页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30900934);国家863计划项目(2006AA10A112);公益性行业(农业)科研专项(3-21油菜菌核病)资助

摘　　要：草酸是核盘菌致病过程中产生的毒素因子。以菌核病菌毒素草酸(3mmol/L)为筛选压,从1000个拟南芥T-DNA插入突变体中筛选出了1个草酸不敏感突变体,命名为275-7。通过拟南芥活体接种对突变体275-7进行菌核病抗性鉴定,与野生型相比,突变体275-7对菌核病的抗性显著增强(P<0.05);荧光定量PCR检测结果表明,275-7中茉莉酸途径的标志基因PDF1.2的表达量是野生型的12倍,而水杨酸途径的标志基因PR1基因的表达量与野生型比较无差异。推测拟南芥突变体275-7可能通过增强水杨酸介导的防卫反应,从而表现出对菌核病的抗性。Oxalic acid is a toxin produced when plant is attacked by Sclerotinia sclerotiorum.An oxalic acid insensitive mutant named 275-7 was screened from the T-DNA insertion mutant library of Arabidopsis thaliana（about 1 000 lines） using 1/2 MS with 3 mmol/L oxalic acid.In vivo leaf inoculation test was undertaken,in order to assess the resistance to Sclerotinia sclerotiorum of mutant 275-7.The result indicated that mutant 275-7 exhibited a higher resistance to S.sclerotiorum than the wild type Arabidopsis（P0.05）.Fluorescence real-time quantitative PCR result showed that the expression of jasmonic acid（JA）-responsive marker gene PDF1.2 in 275-7 were significantly up-regulated（12 fold） in comparison with the wild type plants,but the expression level of salicylic acid（SA）-responsive marker gene PR1 was not significantly different from the wild type.This suggested that the enhanced resistance to S.sclerotiorum in mutant 275-7 might be mediated through JA signal pathway.

关 键 词：拟南芥 突变体 菌核病 抗性 草酸 

分 类 号：S432.44[农业科学—植物病理学]