4种附子配伍方给药后大鼠血浆中3个乌头类生物碱的药动学比较  

Comparative pharmacokinetics of aconitine,mesaconitine and hypaconitine in rats after oral administration of four decoctions composed with Radix Aconiti Lateralis

作　　者：何雷萍[1,2] 狄斌[1] 杜迎翔[1,2] 严方[1] 刘华清[1] 

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室 [2]中国药科大学分析化学教研室,南京210009

出　　处：《中国药科大学学报》2010年第1期55-59,共5页Journal of China Pharmaceutical University

摘　　要：目的:研究大鼠灌胃给予含附子汤剂后乌头碱、新乌头碱和次乌头碱的药动学。方法:4组大鼠分别灌胃给予四逆汤(药液a)、附子水煎液(药液b)、附子加炙甘草水煎液(药液c)及附子加干姜水煎液(药液d),用LC-MS-MS方法测定大鼠血浆中乌头碱、新乌头碱和次乌头碱的浓度,采用DAS2.0计算药动学参数。结果:4种不同配伍方给药后,乌头碱、新乌头碱和次乌头碱的药动学参数有差异。与附子水煎液比,炙甘草与附子配伍,使乌头碱、新乌头碱和次乌头碱3个成分的tmax延迟,MRT0-t延长,且血药浓度-时间曲线出现多峰现象;由干姜及炙甘草配伍附子的四逆汤给药后tmax延迟,MRT0-t缩短。结论:药材配伍对相同成分的药动学行为有影响。Aim：To study pharmacokinetics of aconitine,mesaconitine and hypaconitine in rats after single oral administration of decoctions composed with Radix Aconiti Lateralis.Methods：Four groups of rats were orally administered four decoctions including decoction a（Sini decoction）,decoction b（decoction composed with Radix Aconiti Lateralis）,decoction c（decoction composed with Radix Aconiti Laterali and Radix et Rhizoma Glycyrrhizae Praeparata Cum Melle）and decoction d（decoction composed with Radix Aconiti Laterali and Rhizoma Zingiberis）,respectively.Quantitative analysis of aconitine,mesaconitine and hypaconitine in rat plasma was achieved using a liquid chromatography-electrospray ionization/tandem mass spectrometry method.Pharmacokinetic parameters were estimated using DAS 2.0.Results：Pharmacokinetic parameters of aconitine,mesaconitine and hypaconitine were different after oral administration of four decoctions according to Radix Aconiti Laterali combined with different herbal medicines.Multiple peaks were observed in plasma concentration-time curve after oral administration of the decoction of herb couple Radix Aconiti Laterali and Radix et Rhizoma Glycyrrhizae Praeparata Cum Melle,and the results showed a delay in tmax and a prolonger in MRT0-t compared with the decoction of Radix Aconiti Lateralis.When Radix Aconiti Lateralis was combined with Radix et Rhizoma Glycyrrhizae Praeparata Cum Melle and Rhizoma Zingiberis at the same time in Sini decoction,tmax was delayed too but MRT0-t was shorten than that of the group of Radix Aconiti Lateralis.Conclusion：The pharmacokinetic parameters of the three compounds obtained in this work shows that the pharmacokinetics of aconitine,mesaconitine and hypaconitine were influenced diversely when Radix Aconiti Laterali was combined with different herbal medicines.

关 键 词：乌头碱 新乌头碱 次乌头碱 药动学 

分 类 号：R917[医药卫生—药物分析学] R969.1[医药卫生—药学]