山药多糖对2型糖尿病大鼠肾病的预防作用研究  

Prevention Effects of Polysaccharides from Dioscorea opposita on Kidney Disease in Rats with Type 2 Diabetes Mellitus

作　　者：杨宏莉[1] 张宏馨[1] 王燕[1] 李兰会[2] 崔杰[3] 王桂珍 曲静[5] 

机构地区：[1]河北大学医学部,保定市071000 [2]河北农业大学动物科技学院,保定市071000 [3]河北景县人民医院,衡水市053500 [4]北京市第三社会福利院,北京市100089 [5]河北省荣军医院,保定市071000

出　　处：《中国药房》2010年第15期1345-1347,共3页China Pharmacy

基　　金：河北省科技技术项目(08206416D);河北中医药局科研计划课题(2007006)

摘　　要：目的:研究山药多糖对2型糖尿病大鼠肾病的预防作用。方法:采用链脲佐菌素(STZ)腹腔注射的方法复制2型糖尿病大鼠模型,用山药多糖干预治疗4周,检测血清胰岛素及胰高血糖素、肾组织胰岛素受体(InsR)、胰岛素受体底物-(lIRS-l)、磷酯酰肌醇3激酶(PI-3K)的表达水平。结果:山药多糖可显著升高模型大鼠胰岛素水平(P<0.01),显著降低胰高血糖素水平(P<0.01),显著升高模型大鼠肾组织InsR、IRS-1、PI-3K表达水平(P<0.05)。结论:山药多糖对2型糖尿病大鼠的治疗作用机制可能与提高糖尿病大鼠肾组织中InsR、IRS-1、PI-3K水平,增加组织对胰岛素的敏感性,改善胰岛素信号传导有关。OBJECTIVE：To investigate prevention effects of polysaccharides from Dioscorea opposita on kidney disease in rats with type 2 diabetes mellitus. METHODS：Type 2 diabetic models were induced by injecting with streptozotocin（STZ）. After 4 weeks of treatment,the expression of glucagon,serum insulin,InsR,IRS-1 and PI-3K in rats were determined. RESULTS：D. opposita can significantly increased the levels of serum insulin（P〈0.01）and the expression of InsR,IRS-1 and PI-3K in model rats（P〈0.05）. It can decrease the level of glucagon（P〈0.01）. CONCLUSION：The pharmaceutical mechanisms that polysacch-arides from D. opposita cures type 2 diabetes mellitus maybe associated with the increase of the levels of InsR,IRS-1 and PI-3K in renal tissue,increase of insulin sensitivity and improvement of insulin signal transduction.

关 键 词：山药多糖 2型糖尿病 胰岛素 胰高血糖素 胰岛素受体 胰岛素受体底物-1 磷酯酰肌醇3激酶 

分 类 号：R285[医药卫生—中药学] R587.1[医药卫生—中医学]