毛萼乙素抑制人正常及肝癌血管生成的作用研究  

Inhibition Effect of Eriocalyxin B against Angiogenesis of Hepatocelluar Carcinoma and Human Normal Endothelial Cell

作　　者：班立丽[1] 张慧[2] 罗敏[2] 杨兵兵[2] 韩宏升[2] 潘敏[2] 李玛琳[2] 

机构地区：[1]昆明医学院第二附属医院药剂科,昆明市650101 [2]昆明医学院省天然药物药理重点实验室,昆明市650031

出　　处：《中国药房》2010年第15期1351-1353,共3页China Pharmacy

摘　　要：目的:研究毛萼乙素对人脐静脉内皮细胞(HUVEC)增殖的影响以及对人肝癌血管生成的影响。方法:采用改良MTT法测试6个浓度的毛萼乙素在体外对原代培养的HUVEC增殖的影响;用人肝癌鸡胚尿囊膜血管复制模型,测定毛萼乙素在器官水平对血管生成的抑制作用。结果:毛萼乙素对HUVEC增殖具有显著的抑制作用,半数抑制浓度(IC50)=7.27μg·mL-1;抑制效应呈剂量和时间依赖性。在剂量为25、50、100μg·mL-1时对人肝癌鸡胚尿囊膜血管面积比的抑制率分别为14.15%、48.72%、60.63%,抑制作用中等;其中剂量在50μg·mL-1时与阳性对照反应停相当(P>0.05)。结论:毛萼乙素对人脐静脉内皮细胞的增殖有显著的抑制作用,并能显著抑制人肝癌细胞诱导的鸡胚尿囊膜血管生成。OBJECTIVE：To study the effect of eriocalyxin B （ER-B） on the proliferation of human umbilical vein endothelial cell （HUVEC） and hepatocelluar carcinoma angiogenesis. METHODS：The effects of ER-B at 6 kinds of concentration on the proliferation of HUVEC cultured in vitro were detected using modified MTT assay. Human hepatic cancer chicken chorioallantoic membrane angiogenesis model was induced to determine the inhibition effect of ER-B against angiogenesis. RESULTS：ER-B showed significant inhibitory effects on the proliferation of HUVEC in a dose and time dependent manner with IC50 about 7.27 μg·mL-1. The moderate inhibition rate of ER-B in dose of 25 μg·mL-1,50 μg·mL-1 and 100 μg·mL-1 to area of human hepatic cancer chicken chorioallantoic membrane angiogenesis were 14.15%,48.72% and 60.63%,respectively. 50 μg·mL-1 ER-B has same effect to positive control. CONCLUSION：ER-B significantly inhibits the proliferation of HUVEC and chicken chorioallantoic membrane angiogenesis induced by human liver cancer cell.

关 键 词：毛萼乙素 人脐静脉内皮细胞 血管生成 肝癌细胞 鸡胚尿囊膜 

分 类 号：R285[医药卫生—中药学] R969.1[医药卫生—中医学]