白花蛇舌草中黄酮类抗肿瘤活性成分的HPLC分析  

Determination of Anticancer Activity Components of Flavonoids from Hedyotis diffusa by HPLC

作　　者：邵振中[1,2] 贾晓斌[1,2] 辛然[1] 金晓勇[1] 陈彦[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室/国家中医药管理局名医名方适宜剂型重点研究室,南京市210028 [2]江苏大学药学院,镇江市212013

出　　处：《中国药房》2010年第15期1394-1396,共3页China Pharmacy

基　　金：江苏省自然科学基金项目(BK2006155);江苏省中医药领军人才项目(2006)

摘　　要：目的:建立一种同时测定白花蛇舌草中黄酮类抗肿瘤活性成分槲皮素和山柰酚含量的方法。方法:采用高效液相色谱(HPLC)法,色谱柱为AlltimaC1(8250mm×4.6mm,5μm),流动相为甲醇(A)-0.5%冰醋酸(梯度洗脱),检测波长为350nm。结果:槲皮素、山柰酚的进样量分别在0.0062～0.2440μg(r=0.9998)、0.0078～0.3106μg(r=0.9999)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为101.84%(RSD=1.79%,n=6)和99.04%(RSD=2.90%,n=6)。结论:本方法简便、准确、重复性好,可用于白花蛇舌草药材的质量控制。OBJECTIVE：To establish the method for the simultaneous determination of anticancer activity components of flavonoids from Hedyotis diffusa,i.e. quercetin,kaempferol. METHODS：HPLC was applied to determine the contents and performed on Alltima C18（250 mm×4.6 mm,5 μm） column. Mobile phase consisted of methanol（A）-0.5% glacial acetic acid,（gradient elution）. The detection wavelength was aet at 350 nm. RESULTS：The linear range of quercetin was 0.006 2～0.244 0 μg（r=0.999 8）and that of kaempferol 0.007 8～0.310 6 μg（r=0.999 9）. The average recovery of quercetin was 101.84%（RSD=1.79%,n=6） and that of kaempferol 99.04%（RSD=2.90%,n=6）. CONCLUSIONS：The method is simple,accurate and reproducible for the quality control of H. diffusa.

关 键 词：白花蛇舌草 槲皮素 山柰酚 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]